脂肪酶的固定化及其在药物合成中的应用进展

doi:10.19586/j.2095-2341.2022.0207

生物技术进展 ›› 2023, Vol. 13 ›› Issue (2): 220-227.DOI: 10.19586/j.2095-2341.2022.0207

脂肪酶的固定化及其在药物合成中的应用进展

周亚梅(), 刘佳, 陆丹, 孔庆新()

重庆化工职业学院制药工程学院，重庆 401228

收稿日期:2022-12-08 接受日期:2023-02-17 出版日期:2023-03-25 发布日期:2023-04-07
通讯作者: 孔庆新
作者简介:周亚梅 E-mail: chm_zhouym@163.com；
基金资助:
重庆市教委科学技术研究计划项目重点项目(KJZD-K202104501);校级科研一般课题(HZY2022-KJ05)

Progress on Lipase Immobilization and its Application in Pharmaceutical Synthesis

Yamei ZHOU(), Jia LIU, Dan LU, Qingxin KONG()

Department of Pharmaceutical Engineering，Chongqing Chemical Industry Vocational College，Chongqing 401228，China

Received:2022-12-08 Accepted:2023-02-17 Online:2023-03-25 Published:2023-04-07
Contact: Qingxin KONG

摘要/Abstract

摘要：

脂肪酶是一种常用的生物催化剂，被广泛应用于医药、食品、生物化工等领域。但游离脂肪酶稳定性差，易受所处的环境影响，重复使用性差，限制了酶催化工业的应用。针对游离脂肪酶在催化领域的不足，酶固定化技术应运而生。脂肪酶经固定后大大提高了其原有的催化活性和稳定性，利用固定化脂肪酶自身的优良性能选择性催化合成所需产物，反应条件温和、收率高、副反应少，工业应用更加广泛。综述了脂肪酶固定化及其在药物合成中的研究和应用进展，并对固定脂肪酶的前景进行了展望，以期对固定化脂肪酶在工业中的应用提供一定参考。

关键词: 脂肪酶, 固定化, 催化, 药物合成

Abstract:

As a commonly used biocatalyst， lipase is widely used in pharmaceutical， food and biochemical applications. However， the application of lipase in enzyme catalysis industry was limited because of its poor stability of free lipase， susceptibility to the environment and poor reusability. In response to the shortcomings of free lipase in the field of catalysis， enzyme immobilization technology was developed. The immobilization of lipases has greatly improved their original catalytic activity and stability and has led to a wider range of industrial applications. The use of immobilized lipases with their own excellent properties selectively catalyses the synthesis of the desired product with mild reaction conditions， high yields and few side reactions. The research and applications of lipase immobilization and its use in pharmaceutical synthesis were reviewed and the future of immobilized lipases was given， which was expected to provide reference for the application of immobilized lipase in industry.

Key words: lipase, immobilization, catalysis, pharmaceutical synthesis

中图分类号:

Q814.2

周亚梅, 刘佳, 陆丹, 孔庆新. 脂肪酶的固定化及其在药物合成中的应用进展[J]. 生物技术进展, 2023, 13(2): 220-227.

Yamei ZHOU, Jia LIU, Dan LU, Qingxin KONG. Progress on Lipase Immobilization and its Application in Pharmaceutical Synthesis[J]. Current Biotechnology, 2023, 13(2): 220-227.

图/表 8

图1 酶的固定化方法

Fig. 1 Enzyme immobilization methods

图2 氧化石墨烯固定化脂肪酶[12]

Fig. 2 Graphene oxide immobilized lipase[12]

图3 磁性微球表面包埋固定脂肪酶[13]注： PEI——聚乙烯亚胺

Fig. 3 Lipase entrapped in molecular cages on magnetic microsphere surface[13]

图4 壳聚糖固定化脂肪酶[14]

Fig. 4 Chitosan immobilized lipase[14]

图5 金属有机框架化合物固定聚乙二醇修饰后的南极假丝酵母脂肪酶A[17]

Fig. 5 The enhanced MOF-immobilized lipase CAL-A with polyethylene glycol[17]

图6 电聚合一步法制备导电聚合物原位固定化酶[23]

Fig. 6 One-step in situ enzyme immobilization via electropolymerization of conductive polymer[23]

图7 微波辅助Novozym 435催化动力学拆分酮咯酸[29]

Fig. 7 Microwave assisted Novozym 435 catalyzed kinetic resolution of ketorol[29]

图8 固定酶动力学拆分（±）-3-羟基-3-苯基丙酸乙酯[32]

Fig. 8 Kinetic resolution of (±) ethyl-3-hydroxy-3-phenyl propanoate by immobilized enzyme[32]

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脂肪酶的固定化及其在药物合成中的应用进展

Progress on Lipase Immobilization and its Application in Pharmaceutical Synthesis

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