Research and Application Progress of CRISPR/Cas Technology in Herbicide⁃resistant Crops Breeding

doi:10.19586/j.2095-2341.2021.0170

Current Biotechnology ›› 2022, Vol. 12 ›› Issue (2): 189-197.DOI: 10.19586/j.2095-2341.2021.0170

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Research and Application Progress of CRISPR/Cas Technology in Herbicide⁃resistant Crops Breeding

Yunyan FEI(), Jun YANG, Dedao JING, Tianzi LIN, Chuang LI, Huafei QIAN, Shengyuan ZENG, Huaxin HAN, Hongbing GONG()

Zhenjiang Institute of Agricultural Sciences in Hilly Region of Jiangsu，Jiangsu Jurong 212400，China

Received:2021-10-20 Accepted:2021-11-12 Online:2022-03-25 Published:2022-03-25
Contact: Hongbing GONG

CRISPR/Cas技术在抗除草剂作物育种中的研究与应用进展

费云燕(), 杨军, 景德道, 林添资, 李闯, 钱华飞, 曾生元, 韩华新, 龚红兵()

江苏丘陵地区镇江农业科学研究所，江苏句容 212400

通讯作者: 龚红兵
作者简介:费云燕 E-mail：suiyiyixinyisi@163.com；
基金资助:
江苏省重点研发项目(BE2021374-4);江苏省农业自主创新项目（CX〔18〕2022）;镇江市重点研发项目(NY2020012)

Abstract

Abstract:

CRISPR/Cas system is a simple， inexpensive， high efficiency， and precise tool for genome editing. It can precise genome modification and speed up breeding process， which has great potential in creating novel germplasm. This paper summarized CRISPR/Cas toolkits as well as their application in herbicide-resistant crops breeding， and briefly introduced the current challenges and future application of CRISPR/Cas technology for developing herbicide-resistant crops. The paper aimed to provide a theoretical basis for generating herbicide-resistant crops with CRISPR/Cas system in the future.

Key words: CRISPR/Cas technology, herbicide-resistant plant breeding, germplasm

摘要：

CRISPR/Cas系统是一种简单、低成本、高效、精准的基因编辑技术，该技术能够进行基因的定向改造，加速新品种培育进程，在种质资源创制中的应用潜力较高。概述了CRISPR/Cas系统的技术原理及其在作物抗除草剂育种中的应用，简要指出了目前CRISPR/Cas技术在抗除草剂种质创制及应用过程中存在的问题及发展方向，以期为今后利用CRISPR/Cas技术创制抗除草剂新种质提供理论依据。

关键词: CRISPR/Cas技术, 抗除草剂作物育种, 种质资源

CLC Number:

Q943.2

Yunyan FEI, Jun YANG, Dedao JING, Tianzi LIN, Chuang LI, Huafei QIAN, Shengyuan ZENG, Huaxin HAN, Hongbing GONG. Research and Application Progress of CRISPR/Cas Technology in Herbicide⁃resistant Crops Breeding[J]. Current Biotechnology, 2022, 12(2): 189-197.

费云燕, 杨军, 景德道, 林添资, 李闯, 钱华飞, 曾生元, 韩华新, 龚红兵. CRISPR/Cas技术在抗除草剂作物育种中的研究与应用进展[J]. 生物技术进展, 2022, 12(2): 189-197.

Figures/Tables 5

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物种	靶基因	编辑工具	修饰方式	参考文献
水稻	ALS	CRISPR/Cas9	G628W	[27]
	ALS	CRISPR/Cas9（CRISPR/Cpf）+供体DNA	W548L、S627I	[33⁃34，37]
	ALS	CBE	A96V	[39⁃41]
	ALS	CBE	P17F	[26]
	ALS	引导编辑	W548L、P171S	[56，58]
	ALS	引导编辑	S624I	[59]
	ALS	引导编辑	S627N	[57]
	ALS	引导编辑	W542L、S621I	[60]
	EPSPS	CRISPR/Cas9+供体DNA	T102I、P106S	[35]
	EPSPS	CBE	T102I	[41]
	ACCase	ABE	C2186R、I1879V、W2125S	[5，50]
	ACCase	引导编辑	D2176G	[56]
	AFB4	CRISPR/Cas9	基因敲除	[25]
	SF3B1	CRISPR/Cas9	K1050E、H1048Q	[28]
	TubA2	ABE	M268T	[52]
西红柿	ALS	CRISPR/Cas9+供体DNA	P186A	[30]
西红柿	ALS	CBE	P186A	[47]
玉米	ALS	CRISPR/Cas9+供体DNA	P165S	[31，36]
玉米	ALS	CBE	P165S、P165A、P165L	[5，45]
小麦	ALS	CBE	P174F、P174S、P174A	[44，48]
小麦	ACCase	CBE	A1992V	[48]
大豆	ALS	CRISPR/Cas9+供体DNA	P178S	[32]
大豆	ALS	CBE	P182S、P182T	[43]
油菜	ALS	CBE	P197F、P197S、P173S、P173L	[24，43，49]
西瓜	ALS	CBE	P190S、P190L	[46]
烟草	ALS	CRISPR/Cas9+供体DNA	W568L、S647T	[29]
烟草	ALS	CBE	P194S、P194L	[43]

物种	靶基因	编辑工具	修饰方式	参考文献
水稻	ALS	CRISPR/Cas9	G628W	[27]
	ALS	CRISPR/Cas9（CRISPR/Cpf）+供体DNA	W548L、S627I	[33⁃34，37]
	ALS	CBE	A96V	[39⁃41]
	ALS	CBE	P17F	[26]
	ALS	引导编辑	W548L、P171S	[56，58]
	ALS	引导编辑	S624I	[59]
	ALS	引导编辑	S627N	[57]
	ALS	引导编辑	W542L、S621I	[60]
	EPSPS	CRISPR/Cas9+供体DNA	T102I、P106S	[35]
	EPSPS	CBE	T102I	[41]
	ACCase	ABE	C2186R、I1879V、W2125S	[5，50]
	ACCase	引导编辑	D2176G	[56]
	AFB4	CRISPR/Cas9	基因敲除	[25]
	SF3B1	CRISPR/Cas9	K1050E、H1048Q	[28]
	TubA2	ABE	M268T	[52]
西红柿	ALS	CRISPR/Cas9+供体DNA	P186A	[30]
西红柿	ALS	CBE	P186A	[47]
玉米	ALS	CRISPR/Cas9+供体DNA	P165S	[31，36]
玉米	ALS	CBE	P165S、P165A、P165L	[5，45]
小麦	ALS	CBE	P174F、P174S、P174A	[44，48]
小麦	ACCase	CBE	A1992V	[48]
大豆	ALS	CRISPR/Cas9+供体DNA	P178S	[32]
大豆	ALS	CBE	P182S、P182T	[43]
油菜	ALS	CBE	P197F、P197S、P173S、P173L	[24，43，49]
西瓜	ALS	CBE	P190S、P190L	[46]
烟草	ALS	CRISPR/Cas9+供体DNA	W568L、S647T	[29]
烟草	ALS	CBE	P194S、P194L	[43]

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Research and Application Progress of CRISPR/Cas Technology in Herbicide⁃resistant Crops Breeding

CRISPR/Cas技术在抗除草剂作物育种中的研究与应用进展

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