Curr. Biotech. ›› 2017, Vol. 7 ›› Issue (2): 102-105.DOI: 10.3969/j.issn.2095-2341.2017.02.03

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Advances on Target Gene Alidation Method of  Plant miRNA

HE Xuancheng, LV Heshu, HUANG Jiefei, MA Lanqing, YANG Mingfeng   

  1. Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China
  • Received:2016-12-07 Online:2017-03-25 Published:2017-01-12

植物miRNA靶基因验证研究进展

何炫程,吕鹤书,黄捷飞,马兰青,杨明峰*   

  1. 北京农学院生物科学与工程学院, 农业部华北都市农业重点实验室, 北京 102206
  • 通讯作者: 杨明峰，副教授，研究方向为植物代谢调控。E-mail:mfyang@bac.ac.cn
  • 作者简介:何炫程，硕士研究生，研究方向为植物代谢调控。E-mail:1375720631@qq.com。
  • 基金资助:
    国家自然科学基金项目(31370674；31300620)；北京市教育委员会科技计划面上项目(KM201410020001)资助。

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Abstract: Plant microRNAs (miRNAs) can recognize the target gene(mRNA) by the principle of complementary pairing and mediate the cleavage or translation inhibition of the target gene, and the mechanism was used to regulate the expression of target genes. In this paper, we reviewed the mechanism of plant miRNA and its target genes, and the research progress of plant miRNA target gene validation methods from two aspects of the function and the experimental system, which was objectived to explore the relationship between miRNA and its target genes.

Key words: plant miRNA, target gene, validation

摘要： 植物microRNA(miRNA)可以利用碱基互补配对原则对靶基因进行识别并介导靶基因（mRNA）的切割或翻译抑制，以此机制来调控靶基因的表达。主要从作用方式及实验体系两大方面入手，综述了植物miRNA与其靶基因的作用机制，以及植物miRNA靶基因验证方法的研究进展,旨在更深入的探究miRNA与其靶基因的作用关系。

关键词: 植物miRNA, 靶基因, 验证

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