Abstract: Minor ginsenoside Compound K (C-K) exhibited remarkable biological activities. In this study, nine fungi were isolated from soil, and their abilities of transform ginsenoside Rb1 for preparation of C-K were studied. A highly efficient ginsenoside Rb1-hydrolyzing fungus Aspergillus niger sp. J7 was screened. The strain converted ginsenoside Rb1 to C-K with the pathway of Rb1→Rd→F2→C-K. The conversion conditions of A. niger sp. J7 was optimized. Under the optimal conditions, ginsenoside Rb1 was converted to C-K completely. The transformation system was enlarged to 200 mL, in which ginsenoside Rb1 was completely converted into C-K after 60 h and the conversion rate was 74.7%. This fungus was expected to be the basis in bioactive C-K preparation.

Key words:

摘要： 稀有人参皂苷Compound K（C-K）具有显著的生物学活性 。从东北农耕土壤中分离出9株真菌，系统研究了其转化 人参皂苷Rb1制备C-K的能力。其中，黑曲霉Aspergillus niger sp. J7能够高效转化人参皂苷Rb1生成C-K，转化 途径为Rb1→Rd→F2→C-K。对黑曲霉J7转化Rb1制备C-K 的条件进行优化，在最优条件下，Rb1可完全转化成C-K 。将该转化体系扩大到200 mL，60 h内可将Rb1完全转化 成C-K，转化率为74.7%。黑曲霉J7为人参皂苷Rb1高效水 解为稀有人参皂苷C-K奠定了基础。

关键词: 人参皂苷, 黑曲霉, 生物转化, &beta, -葡萄糖苷酶