Curr. Biotech. ›› 2016, Vol. 6 ›› Issue (1): 47-51.DOI: 10.3969/j.issn.2095-2341.2016.01.10

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Isolation and Identification of Bacillus sp. Strain BRC-XYT1 and its Toxicity to Monochamus alternatus Hope

XIONG Yue-ting, XIA Feng, HUANG Zhi-cheng, LIU Li-qin, WENG Xiao-qian, WU Song-qing   

  1. 1.College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2.Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    3.Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2015-11-24 Revised:2015-12-21 Online:2016-01-25 Published:2016-03-31

芽孢杆菌BRC-XYT1菌株的分离和鉴定及其对松墨天牛（Monochamus alternatus Hope）的杀虫活性

熊悦婷1,夏枫2,黄志成2,刘丽琴2,翁小倩2,吴松青3*   

  1. 1.福建农林大学植物保护学院, 福州 350002;
    2.福建农林大学, 生物农药与化学生物学教育部重点实验室, 福州 350002;
    3.福建农林大学林学院, 福州 350002
  • 通讯作者: 吴松青，讲师，博士，主要从事微生物农药研究。E-mail: dabinyang@126.com
  • 作者简介:熊悦婷，本科生，主要从事微生物农药研究。E-mail:xyt941122@126.com。
  • 基金资助:

    国家林业公益性行业科研专项（201304401）；福建农林大学重点项目建设专项（6112C035003）；国家大学生创新创业计划项目（201510389013；201510389018；201510389023）资助。

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Abstract: In this study, strain BRC-XYT1 with cytotoxic activity to Monochamus alternatus Hope larva, was isolated from withered woods. Based on the morphological observation, biochemical and physiological characterization and phylogenetic analysis of its 16S rDNA gene sequence, BRC-XYT1 was identied as one of the strains of Bacillus sp.. When tested with drug sensitive test, strain BRC-XYT1 possessed different levels of sensitivity to a number of common medicine. Besides, strain BRC-XYT1 showed signicant insecticidal activity to Monochamus alternatus Hope larva based on the bioassay data. This study reserved new resource for controlling pine wilt disease and it also provided a theoretical base for the effective development of novel biological pest control agents.

Key words: Bacillus sp., isolation and identification, Monochamus alternatus Hope, insecticidal activity

摘要： 从枯死木中分离出一株对松墨天牛（Monochamus alternatus Hope）幼虫有毒力作用的菌株BRC-XYT1，基于形态学鉴定、生理生化反应、16S rDNA系统发育分析，鉴定该菌株为芽孢杆菌属（Bacillus）的一个菌种。经药敏分析测试显示，其对于一些常见药物有着不同程度的敏感性。此外，基于生物测定数据，该菌株对松墨天牛幼虫表现出显著的杀虫活性。研究结果为控制松材线虫病(Bursaphelenchus xylophilus)储备了新资源，为开拓新型微生物农药提供了理论基础。

关键词: 芽孢杆菌, 分离鉴定, 松墨天牛, 杀虫活性

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