Abstract: In order to explore the extraction technology of GABA from fermentation broth with glutamic acid as substrate, membrane filtration associated with ion\|exchange adsorption and desorption process were studied in this paper, and followed with decolorization by using active carbon. The initial trans\|membrane pressure is 0.85 bar during the filtration process, and the temperature was controlled at 38℃. With QY-021-a strong acid cation exchange resin as packing in ion\|exchange column, adsorption-desorption process was exerted at room temperature. After concentrated by rotary evaporation, decolorization was performed using 15% (carbon/solids in the solution) activate carbon, with temperature fixed at 65℃, stirring for 40 min. Results showed that the average flux was 128 L/m2·h, and concentration ratio was 33.3. Samplings to detect membrane permeate showed that the yield of GABA was 97.7%, which indicated that there was no GABA retention of interception and adsorption on microfiltration membrane surface and fouling layer. After collecting 5 BV permeate to calculate the yield of ion-exchange process, results showed that the yield was 92.8%, decolorization ratio was 94.2% by using activate carbon B, and the GABA yield was 99.2%. The total yield of the whole process was up to 85%, which indicated that this process has the prospects for industrial application, and further improvements of yield could be achieved by optimized ion exchange processes and equipment.

Key words: GABA, glutamic acid, broth, membrane seperation, ion-exchange

摘要： 为掌握从谷氨酸发酵液提取生物基尼龙聚丁内酰胺单体γ-氨基丁酸（GABA）的技术，本文研究了膜过滤联合离子交换吸附和洗脱的工艺，并且使用活性炭进行脱色。膜过滤过程中初始大跨膜压差为0.85 bar，过滤温度控制在38℃；离子交换过程采用QY\|021\|a强酸型阳离子交换树脂，常温下吸附-洗脱的操作方法；经旋转蒸发浓缩后，添加15%（碳对固形物含量之比）活性炭，温度为65℃，搅拌40 min脱色。结果表明：平均膜通量为128 L/m2·h，浓缩倍数为33.3倍。经取样检测膜透过液样品，浓缩液样品计算GABA收率为97.7%，微滤膜以及膜表面污染物上无GABA截留和吸附截留；离子交换经5 BV洗脱后计算收率，吸附-洗脱过程的收率为92.8%；使用活性碳B的脱色率为94.2%，GABA收率为99.2%。该工艺的总收率为85%，该工艺具有工业化应用前景，并且可以通过优化离子交换工艺和设备进一步提高收率。

关键词: &gamma, -氨基丁酸, 谷氨酸, 发酵液, 膜分离, 离子交换