Abstract: Corn fiber, a byproduct of corn starch processing, is a potential unexpensive bioresource for butanol production. Butanol production from corn fiber using Clostridium beijerinckii NCIMB 8052 was investigated in this study. Corn fiber was firstly pretreated by 0.5% sulfuric acid with solid\|to\|liquid ratio of 1：8 for 20 min under the optimal temperature 140℃，followed by enzymatic hydrolysis at glucoamylase loading of 200 IU/g（dry material）and xylanase loading of 1.0 IU/g （dry material）. The starch and hemicellulose were mostly hydrolyzed into fermentable sugars including glucose，xylose and arabinose，resulting in a total fermentable sugars concentration of 50.46 g/L. Then the hydrolysate of corn fiber was detoxified by 1.0% active carbon in order to remove the inhibitors of butanol fermentation. Batch fermentation using the detoxified hydrolysate of corn fiber showed that 9.72 g/L of butanol and 14.09 g/L of total solvents were produced at the end of the fermentation period，and the sugar-to-solvents ratio was 35.1%. These results above suggested that it is a feasible strategy for butanol production by corn fiber fermentation using Clostridium beijerinckii NCIMB 8052.

Key words: corn fiber, butanol fermentation, Clostridium beijerinckii NCIMB 8052

摘要： 玉米皮作为玉米淀粉加工的副产物，是一种可用于生产液体燃料的潜在廉价优质的生物质资源。本文以玉米皮为原料，对拜氏梭菌发酵生产丁醇进行了研究。实验结果表明，玉米皮首先在最优的预处理温度140℃下使用0.5%硫酸水溶液以固液比1∶8处理20 min，再添加200 IU/g底物糖化酶、1.0 IU/g底物木聚糖酶进行酶解，可以使原料中的淀粉和半纤维素转化为可发酵糖，此时水解液中的总糖浓度为50.46 g/L。然后使用1.0%的活性炭对水解液进行脱毒处理以去除发酵抑制物，再进行丁醇发酵，丁醇产量为9.72 g/L，总溶剂产量可达14.09 g/L，糖醇转化率为35.1%。上述研究结果证明玉米皮作为一种粮食加工废弃物用于液体燃料丁醇的生产在技术上是完全可行的。

关键词: 玉米皮, 丁醇发酵, 拜氏梭菌