Research Progress of CRISPR/Cas9 Gene Editing Technology in Livestock and Poultry Breeding

doi:10.19586/j.2095-2341.2024.0021

Current Biotechnology ›› 2024, Vol. 14 ›› Issue (4): 529-536.DOI: 10.19586/j.2095-2341.2024.0021

• Reviews • Previous Articles Next Articles

Research Progress of CRISPR/Cas9 Gene Editing Technology in Livestock and Poultry Breeding

Mingyang JIA¹(), Lei WANG¹, Junfeng CHEN², Jiaqing ZHANG², Xiangzhou YAN², Baosong XING²(), Jing WANG²()

^1.College of Animal Science and Veterinary Medcine，Henan Institute of Science and Technology，Henan Xinxiang 453003，China
^2.Henan Engineering Research Center for Breeding Swine Industry，Henan Key Laboratory of Farm Animal Breeding and Nutritional Regulation，Institute of Animal Husbandry，Henan Academy of Agricultural Sciences，Zhengzhou 450002，China

Received:2024-02-06 Accepted:2024-05-20 Online:2024-07-25 Published:2024-08-07
Contact: Baosong XING,Jing WANG

CRISPR/Cas9基因编辑技术在畜禽育种中的研究进展

贾名扬¹(), 王磊¹, 陈俊峰², 张家庆², 闫祥洲², 邢宝松²(), 王璟²()

^1.河南科技学院动物科技学院，河南新乡 453003
^2.河南省农业科学院畜牧研究所，河南省畜禽繁育与营养调控重点实验室，河南省生猪种业工程研究中心，郑州 450002

通讯作者: 邢宝松,王璟
作者简介:贾名扬 E-mail: jia1my@163.com
基金资助:
国家重点研发计划项目(2021YFD1301200);河南省优秀青年科学基金项目(222300420051);河南省重点研发与推广专项(212102110010);河南省农业科学院科技创新团队项目(2024TD22)

Abstract

Abstract:

CRISPR/Cas9 is an efficient and accurate gene editing technology， which is widely used in the field of livestock and poultry gene editing. This paper introduced the research progress and application of CRISPR/Cas9 technology in breeding of pig， cow， sheep and poultry， summarized the problems of its application in breeding， and prospected its future development trend， in order to provide reference for the future application of the technology in the field of livestock and poultry breeding.

Key words: CRISPR/Cas9 system, gene editing, livestock breeding

摘要：

CRISPR/Cas9是一种高效、精准的基因编辑技术，在畜禽基因编辑领域已取得了广泛应用。简述了CRISPR/Cas9技术在猪、牛、羊及禽类遗传育种方面的研究进展和应用情况，总结了该技术在育种应用方面所面临的问题，并对其未来发展趋势进行了展望，以期为未来该技术在畜禽育种领域的应用提供参考。

关键词: CRISPR/Cas9系统, 基因编辑, 畜禽育种

CLC Number:

Mingyang JIA, Lei WANG, Junfeng CHEN, Jiaqing ZHANG, Xiangzhou YAN, Baosong XING, Jing WANG. Research Progress of CRISPR/Cas9 Gene Editing Technology in Livestock and Poultry Breeding[J]. Current Biotechnology, 2024, 14(4): 529-536.

贾名扬, 王磊, 陈俊峰, 张家庆, 闫祥洲, 邢宝松, 王璟. CRISPR/Cas9基因编辑技术在畜禽育种中的研究进展[J]. 生物技术进展, 2024, 14(4): 529-536.

References 64

1	赵美威,段承俐,刘江.基于类转录激活因子效应物(TALEs)的基因组定点操控技术[J].动物学研究,2013,34(5):509-518.
	ZHAO M W, DUAN C L, LIU J. Transcription activator-like effectors(TALEs)based genome engineering[J]. Zool. Res., 2013, 34(5): 509-518.
2	LAI L, KANG J X, LI R, et al.. Generation of cloned transgenic pigs rich in omega-3 fatty acids[J]. Nat. Biotechnol., 2006, 24(4): 435-436.
3	朱秋宇,胡兰.动物机体中肌肉生长抑制素基因的研究进展[J].现代畜牧兽医,2014(9):51-55.
	ZHU Q Y, HU L. Research progress of Myostatin gene in animal[J]. Mod. J. Anim. Husb. Vet. Med., 2014(9): 51-55.
4	WANG K, OUYANG H, XIE Z, et al.. Efficient generation of myostatin mutations in pigs using the CRISPR/Cas9 system[J/OL]. Sci. Rep., 2015, 5: 16623[2024-05-31]. .
5	WANG K, TANG X, XIE Z, et al.. CRISPR/Cas9-mediated knockout of myostatin in Chinese indigenous Erhualian pigs[J]. Transgenic Res., 2017, 26(6): 799-805.
6	ZHU X X, ZHAN Q M, WEI Y Y, et al.. CRISPR/Cas9-mediated MSTN disruption accelerates the growth of Chinese Bama pigs[J]. Zuchthygiene, 2020, 55(10): 1314-1327.
7	彭定威,李瑞强,曾武,等.编辑MSTN半胱氨酸节基元促进两广小花猪肌肉生长[J].遗传,2021,43(3):261-270.
	PENG D W, LI R Q, ZENG W, et al.. Editing the cystine knot motif of MSTN enhances muscle development of Liang Guang Small Spotted pigs[J]. Hereditas, 2021, 43(3): 261-270.
8	王煜,宋瑞高,赵建国,等.碱基编辑器介导的猪IGF2基因高效定点突变[J].中国畜牧兽医,2020,47(11):3427-3435.
	WANG Y, SONG R G, ZHAO J G, et al.. Efficient site-directed mutation of porcine IGF2 gene via base editors[J]. China Anim. Husb. Vet. Med., 2020, 47(11): 3427-3435.
9	XIANG G, REN J, HAI T, et al.. Editing porcine IGF2 regulatory element improved meat production in Chinese Bama pigs[J]. Cell. Mol. Life Sci., 2018, 75(24): 4619-4628.
10	WANG H, SHEN L, CHEN J, et al.. Deletion of CD163 exon 7 confers resistance to highly pathogenic porcine reproductive and respiratory viruses on pigs[J]. Int. J. Biol. Sci., 2019, 15(9): 1993-2005.
11	BURKARD C, OPRIESSNIG T, MILEHAM A J, et al.. Pigs lacking the scavenger receptor cysteine-rich domain 5 of CD163 are resistant to porcine reproductive and respiratory syndrome virus 1 infection[J]. J. Virol., 2018, 92(16): 415-418.
12	CHEN J, WANG H, BAI J, et al.. Generation of pigs resistant to highly pathogenic-porcine reproductive and respiratory syndrome virus through gene editing of CD163 [J]. Int. J. Biol. Sci., 2019, 15(2): 481-492.
13	WANG Y, BI D, QIN G, et al.. Cytosine base editor (hA3A-BE3-NG)-mediated multiple gene editing for pyramid breeding in pigs[J/OL]. Front. Genet., 2020, 11: 592623[2024-05-31]. .
14	赵为民,王慧利,曹少先,等.猪CD163基因的单碱基编辑研究[J].畜牧兽医学报,2022,53(4):1041-1050.
	ZHAO W M, WANG H L, CAO S X, et al.. The study of base editing of porcine CD163 gene[J]. Acta Vet. Zootechnica Sin., 2022, 53(4): 1041-1050.
15	SONG R, WANG Y, ZHENG Q, et al.. One-step base editing in multiple genes by direct embryo injection for pig trait improvement[J]. Sci. China Life Sci., 2022, 65(4): 739-752.
16	XU K, ZHOU Y, MU Y, et al.. CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance[J/OL]. eLife, 2020, 9: e57132[2024-05-31]. .
17	REN J, HAI T, CHEN Y, et al.. Improve meat production and virus resistance by simultaneously editing multiple genes in livestock using Cas12i^Max [J]. Sci. China Life Sci., 2024, 67(3): 555-564.
18	王妍鳕,任亭亭,孙跃峰,等.利用CRISPR/Cas9系统构建SBNO2基因敲除细胞系及其功能研究[J].甘肃农业大学学报,2021,56(1):22-28.
	WANG Y X, REN T T, SUN Y F, et al.. Construction of SBNO2 knockout cell lines using CRISPR/Cas9 system and its function evaluation[J]. J. Gansu Agric. Univ., 2021, 56(1): 22-28.
19	张林,吴金恩,任玫,等. RALY基因敲除PK-15细胞系的构建及对口蹄疫病毒复制的影响[J].中国兽医科学,2023,53(9):1115-1121.
	ZHANG L, WU J N, REN M, et al.. Construction of RALY gene knockout PK-15 cell line and its effect on replication of foot-and-mouth disease virus[J]. Chin. Vet. Sci., 2023, 53(9): 1115-1121.
20	XIE Z, JIAO H, XIAO H, et al.. Generation of pRSAD2 gene knock-in pig via CRISPR/Cas9 technology[J/OL]. Antivir. Res., 2020, 174: 104696[2024-05-31]. .
21	QI C, PANG D, YANG K, et al.. Generation of PCBP1-deficient pigs using CRISPR/Cas9-mediated gene editing[J/OL]. iScience, 2022, 25(10): 105268[2024-05-31]. .
22	BERG F, GUSTAFSON U, ANDERSSON L. The uncoupling protein 1 gene (UCP1) is disrupted in the pig lineage: a genetic explanation for poor thermoregulation in piglets[J/OL]. PLoS Genet., 2006, 2(8): e129[2024-05-31]. .
23	ZHENG Q, LIN J, HUANG J, et al.. Reconstitution of UCP1 using CRISPR/Cas9 in the white adipose tissue of pigs decreases fat deposition and improves thermogenic capacity[J]. Proc. Natl. Acad. Sci. USA, 2017, 114(45): 9474-9482.
24	LIANG X, LAN J, XU M, et al.. Impact of KIT editing on coat pigmentation and fresh meat color in Yorkshire pigs[J]. CRISPR J., 2022, 5(6): 825-842.
25	吴珊珊,王学侨,王鑫,等. MSTN基因编辑鲁西牛屠宰性状与肉用品质分析[J].农业生物技术学报,2023,31(1):87-97.
	WU S S, WANG X Q, WANG X, et al.. Analysis of slaughter traits and meat quality of MSTN gene-edited Luxi cattle(Bos taurus)[J]. J. Agric. Biotechnol., 2023, 31(1): 87-97.
26	GIM G M, KWON D H, EOM K H, et al.. Production of MSTN-mutated cattle without exogenous gene integration using CRISPR-Cas9[J/OL]. Biotechnol. J., 2022, 17(7): e2100198[2024-05-31]. .
27	CRISPO M, MULET A P, TESSON L, et al.. Efficient generation of myostatin knock-out sheep using CRISPR/Cas9 technology and microinjection into zygotes[J/OL]. PLoS ONE, 2015, 10(8): e0136690[2024-05-31]. .
28	WANG X, NIU Y, ZHOU J, et al.. Multiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep[J/OL]. Sci. Rep., 2016, 6: 32271[2024-05-31]. .
29	姚旭东.单碱基编辑哈萨克羊MSTN基因的研究[D].石河子:石河子大学,2021.
30	VAL C H, DE OLIVEIRA M C, LACERDA D R, et al.. SOCS2 modulates adipose tissue inflammation and expansion in mice[J/OL]. J. Nutr. Biochem., 2020, 76: 108304[2024-05-31]. .
31	ZHOU S, CAI B, HE C, et al.. Programmable base editing of the sheep genome revealed No genome-wide off-target mutations[J/OL]. Front. Genet., 2019, 10: 215[2024-05-31]. .
32	SILAEVA Y Y, KUBEKINA M V, BRUTER A V, et al.. Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout[J/OL]. E3S Web Conf., 2020, 176: 1006[2024-05-31]. .
33	ZHOU W, WAN Y, GUO R, et al.. Generation of beta-lactoglobulin knock-out goats using CRISPR/Cas9[J/OL]. PLoS ONE, 2017, 12(10): e0186056[2024-05-31]. .
34	TIAN H, LUO J, ZHANG Z, et al.. CRISPR/Cas9-mediated stearoyl-CoA desaturase 1 (SCD1) deficiency affects fatty acid metabolism in goat mammary epithelial cells[J]. J. Agric. Food Chem., 2018, 66(38): 10041-10052.
35	TAN D X, MANCHESTER L C, ESTEBAN-ZUBERO E, et al.. Melatonin as a potent and inducible endogenous antioxidant: synthesis and metabolism[J]. Molecules, 2015, 20(10): 18886-18906.
36	MA T, TAO J, YANG M, et al.. An AANAT/ASMT transgenic animal model constructed with CRISPR/Cas9 system serving as the mammary gland bioreactor to produce melatonin-enriched milk in sheep[J/OL]. J. Pineal Res., 2017, 63(1): 12406 [2024-05-31]. .
37	GAO Y, WU H, WANG Y, et al.. Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects[J/OL]. Genome Biol., 2017, 18(1): 13[2024-05-31]. .
38	WORKMAN A M, HEATON M P, VANDER LEY B L, et al.. First gene-edited calf with reduced susceptibility to a major viral pathogen[J/OL]. PNAS Nexus, 2023, 2(5): pgad125[2024-05-31]. .
39	HU S, YANG M, POLEJAEVA I. 360 double knockout of goat myostatin and prion protein gene using clustered regularly interspaced short palindromic repeat CRISPR/Cas9 systems[J/OL]. Reprod. Fertil. Dev., 2015, 27(1): 268[2024-05-31]. .
40	MENCHACA A, MULET A P, DOS SANTOS NETO P C, et al.. CRISPR in sheep: a southern perspective[J]. Transgenic Res., 2018, 27(5): 469-470.
41	李冠纬.单碱基基因编辑系统介导的FGF5基因敲除绒山羊的创制与评价[D].杨凌:西北农林科技大学,2020.
42	丁一格.ABEs介导的FecB基因突变滩羊的制备[D].杨凌:西北农林科技大学,2020.
43	BHATTACHARYA T K, SHUKLA R, CHATTERJEE R N, et al.. Comparative analysis of silencing expression of myostatin (MSTN) and its two receptors (ACVR2A and ACVR2B) genes affecting growth traits in knock down chicken[J/OL]. Sci. Rep., 2019, 9(1): 7789[2024-05-31]. .
44	KIM G D, LEE J H, SONG S, et al.. Generation of myostatin-knockout chickens mediated by D10A-Cas9 nickase[J]. FASEB J., 2020, 34(4): 5688-5696.
45	ZIMMERMANN R, STRAUSS J G, HAEMMERLE G, et al.. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase[J]. Science, 2004, 306(5700): 1383-1386.
46	YANG X, LU X, LOMBÈS M, et al.. The G(0)/G(1) switch gene 2 regulates adipose lipolysis through association with adipose triglyceride lipase[J]. Cell Metab., 2010, 11(3): 194-205.
47	PARK T S, PARK J, LEE J H, et al.. Disruption of G(0)/G(1) switch gene 2 (G0S2) reduced abdominal fat deposition and altered fatty acid composition in chicken[J]. FASEB J., 2019, 33(1): 1188-1198.
48	LEE H J, YOON J W, JUNG K M, et al.. Targeted gene insertion into Z chromosome of chicken primordial germ cells for avian sexing model development[J]. FASEB J., 2019, 33(7): 8519-8529.
49	LEE H J, LEE K Y, PARK Y H, et al.. Acquisition of resistance to avian leukosis virus subgroup B through mutations on tvb cysteine-rich domains in DF-1 chicken fibroblasts[J/OL]. Vet. Res., 2017, 48(1): 48[2024-05-31]. .
50	LEE H J, PARK K J, LEE K Y, et al.. Sequential disruption of ALV host receptor genes reveals no sharing of receptors between ALV subgroups A, B, and J[J/OL]. J. Anim. Sci. Biotechnol., 2019, 10: 23[2024-05-31]. .
51	KOSLOVÁ A, TREFIL P, MUCKSOVÁ J, et al.. Precise CRISPR/Cas9 editing of the NHE1 gene renders chickens resistant to the J subgroup of avian leukosis virus[J]. Proc. Natl. Acad. Sci. USA, 2020, 117(4): 2108-2112.
52	战彦韬.应用CRISPR/Cas9敲低DEF细胞HMGB1基因对鸭坦布苏病毒复制的影响[D].泰安:山东农业大学,2020.
53	ADIKUSUMA F, PILTZ S, CORBETT M A, et al.. Large deletions induced by Cas9 cleavage[J]. Nature, 2018, 560(7717): 8-9.
54	CULLOT G, BOUTIN J, TOUTAIN J, et al.. CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations[J/OL]. Nat. Commun., 2019, 10(1): 1136[2024-05-31]. .
55	HAAPANIEMI E, BOTLA S, PERSSON J, et al.. CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response[J]. Nat. Med., 2018, 24(7): 927-930.
56	IHRY R J, WORRINGER K A, SALICK M R, et al.. p53 inhibits CRISPR-Cas9 engineering in human pluripotent stem cells[J]. Nat. Med., 2018, 24(7): 939-946.
57	LEIBOWITZ M L, PAPATHANASIOU S, DOERFLER P A, et al.. Chromothripsis as an on-target consequence of CRISPR-Cas9 genome editing[J]. Nat. Genet., 2021, 53(6): 895-905.
58	HU J H, MILLER S M, GEURTS M H, et al.. Evolved Cas9 variants with broad PAM compatibility and high DNA specificity[J]. Nature, 2018, 556(7699): 57-63.
59	ZETSCHE B, GOOTENBERG J S, ABUDAYYEH O O, et al.. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system[J]. Cell, 2015, 163(3): 759-771.
60	XU C, ZHOU Y, XIAO Q, et al.. Programmable RNA editing with compact CRISPR-Cas13 systems from uncultivated microbes[J]. Nat. Meth., 2021, 18(5): 499-506.
61	HILLARY V E, CEASAR S A. A review on the mechanism and applications of CRISPR/Cas9/Cas12/Cas13/Cas14 proteins utilized for genome engineering[J]. Mol. Biotechnol., 2023, 65(3): 311-325.
62	PORTO E M, KOMOR A C. In the business of base editors: evolution from bench to bedside[J/OL]. PLoS Biol., 2023, 21(4): e3002071[2024-05-31]. .
63	HUANG J, LIN Q, FEI H, et al.. Discovery of deaminase functions by structure-based protein clustering[J]. Cell, 2023, 186(15): 3182-3195.
64	ALTAE-TRAN H, KANNAN S, SUBERSKI A J, et al.. Uncovering the functional diversity of rare CRISPR-Cas systems with deep terascale clustering[J/OL]. Science, 2023, 382(6673): eadi1910[2024-05-31]. .

[1]	Yiyang LI, Zhizheng ZHOU, Shufei WANG, Boya LIU, Yufei LIU, Xiaoyan LI, Hongshu SUI, Dongwei LIU. Application and Prospect of CRISPR/Cas9 Gene Editing Technology in Disease Treatment [J]. Current Biotechnology, 2025, 15(1): 35-42.
[2]	Guang HU, Zhi WANG, Wei FU, Yuting SHI, Shanshan CHEN, Liang LUO, Shuang WEI. Establishment of Detection Method Based on TaqMan Real-time Fluorescence Quantitative PCR Technology for OsWx-edited Rice [J]. Current Biotechnology, 2025, 15(1): 86-92.
[3]	Jing WANG, Haitao GUAN, Xiaolei ZHANG, Baohuai WANG, Baohai LIU, Hongtao WEN. Detection Dynamic and Development Tendency of Agricultural Gene Editing Products [J]. Current Biotechnology, 2024, 14(5): 712-723.
[4]	Kehao CAO, Junli ZHU, Huashan HE, Weizhuo XU. Impact of the Fourth Modifications of Patent Laws on Biotechnology Patent Applications and Industry Development [J]. Current Biotechnology, 2023, 13(5): 663-670.
[5]	Ali WANG, Jiangdong LIU. Research Progress on the CRISPR/Cas System in Zebrafish [J]. Current Biotechnology, 2023, 13(4): 485-491.
[6]	Siyu GAI, Ziqi CHEN, Hanchao XIA, Rengui ZHAO, Xiangguo LIU. Research Progress of CRISPR/Cas9 Technology in Plant Promoter Editing [J]. Current Biotechnology, 2023, 13(3): 321-328.
[7]	Hui SUN, Chunyi ZHANG, Ling JIANG. Progress of Plant Molecular Farming in Pharmaceutical Use [J]. Current Biotechnology, 2023, 13(1): 65-71.
[8]	Yang YANG, Fenglin WANG, De LIU, Yuanyuan LUO, Jianhua ZHU. Research Progress of CRISPR‍⁃‍Cas9 Technology on the Production of Plant Secondary Metabolites [J]. Current Biotechnology, 2022, 12(6): 806-816.
[9]	Kun YU, Jiaqi XUE, Jinkuan WANG, Yongtao YU. Research Progress on Application of CRISPR/Cas9 Gene Editing Technique in Filamentous Fungi [J]. Current Biotechnology, 2022, 12(5): 696-704.
[10]	Weisong GAO, Jinping DOU, Shuang WEI, Xingjian LIU, Zhifang ZHANG, Yinyu LI. Classification and Research Status of CRISPR/Cas Systems [J]. Current Biotechnology, 2022, 12(4): 532-538.
[11]	Xing DANG, Binwei ZHI, Kehao CAO, Tingting LIU, Biao CHEN, Yuanjie DING. Patent Analysis on Genetically Modified Maize Biological Breeding Technology and Development Suggestions [J]. Current Biotechnology, 2022, 12(4): 614-622.
[12]	Yaohui HUANG, Yijie WANG, Litao YANG, Yue JIAO, Zhongwen FU. Safety Management of the Crop Produced by New Breeding Techniques [J]. Current Biotechnology, 2022, 12(2): 198-204.
[13]	Mengyu WANG, Haoqian WANG, Xujing WANG, Zhixing WANG. Research Progress of Gene Editing Products Detection Technology [J]. Current Biotechnology, 2021, 11(4): 438-445.
[14]	GERILEQIMUGE, NIU Zhenfeng, DONG Dan, ZHANG Taotao, ZHENG Rong. Application Progress of CRISPR-Cas System in Microbial Research [J]. Current Biotechnology, 2021, 11(3): 253-259.
[15]	CAO Haohao1, ZHANG Hongbing1*, XUE Xifa1, LI Zuoqun2, YAN Hongbo1, LI Huixuan1. Application Progress of New Gene Editing Technology in Single Cell Microalgae [J]. Curr. Biotech., 2021, 11(1): 9-15.

Research Progress of CRISPR/Cas9 Gene Editing Technology in Livestock and Poultry Breeding

CRISPR/Cas9基因编辑技术在畜禽育种中的研究进展

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

References 64

Related Articles 15

Recommended Articles

Metrics

Comments