Research Progress on Plant Endophytic Nitrogen⁃fixing Bacteria and Their Nitrogen Fixation Mechanism

doi:10.19586/j.2095-2341.2021.0126

Current Biotechnology ›› 2022, Vol. 12 ›› Issue (1): 17-26.DOI: 10.19586/j.2095-2341.2021.0126

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Research Progress on Plant Endophytic Nitrogen⁃fixing Bacteria and Their Nitrogen Fixation Mechanism

Yuhu WANG(), Mingmin ZHAO, Hongli ZHENG()

College of Horticulture and Plant Protection，Inner Mongolia Agricultural University，Hohhot 010019，China

Received:2021-07-02 Accepted:2021-09-03 Online:2022-01-25 Published:2022-01-26
Contact: Hongli ZHENG

植物内生固氮菌及其固氮机理研究进展

王玉虎(), 赵明敏, 郑红丽()

内蒙古农业大学园艺与植物保护学院，呼和浩特 010019

通讯作者: 郑红丽
作者简介:王玉虎 E-mail：wyh1005433055@163.com；
基金资助:
内蒙古农业大学高层次人才引进科研启动项目(NDGCC2016-23)

Abstract

Abstract:

Nitrogen is an essential element for plant growth. Plant endophytic nitrogen-fixing bacteria can not only produce nitrogen in the plant body for plant utilization， but also play an active role in the natural nitrogen cycle， which has important significance for the sustainable development of agriculture. The research on endogenous nitrogen-fixing bacteria has gradually become research hotspot in recent years. This article systematically reviewed the research progress of plant endophytic nitrogen-fixing bacteria from many aspects， such as the discovery of plant endophytic nitrogen-fixing bacteria， crop symbiosis， invasion pathways， nitrogen-fixing mechanism， and growth-promoting mechanism. In addition， new research ideas of plant endophytic nitrogen-fixing bacteria and some unresolved problems were discussed. This paper was expected to provide reference for research on plant endophytic nitrogen-fixing bacteria and biological nitrogen fixation.

Key words: endophytic nitrogen-fixing bacteria, nitrogenase, nitrogen fixation mechanism, biological nitrogen fixation

摘要：

氮素是植物生长必不可少的元素，植物内生固氮菌不仅能够在植物体内产生氮素以供植物利用，而且在自然界氮素循环过程中发挥积极作用，对农业可持续发展具有重要意义。近年来，植物内生固氮菌逐渐成为研究热点。由植物内生固氮菌的发现、作物共生、侵入途径、固氮机理、促生作用机制等方面系统地综述了植物内生固氮菌的研究进展，探讨了植物内生固氮菌新的研究思路以及一些尚未解决的问题，以期为植物内生固氮菌及生物固氮研究提供参考。

关键词: 内生固氮菌, 固氮酶, 固氮机理, 生物固氮

CLC Number:

Q939.11+3

Yuhu WANG, Mingmin ZHAO, Hongli ZHENG. Research Progress on Plant Endophytic Nitrogen⁃fixing Bacteria and Their Nitrogen Fixation Mechanism[J]. Current Biotechnology, 2022, 12(1): 17-26.

王玉虎, 赵明敏, 郑红丽. 植物内生固氮菌及其固氮机理研究进展[J]. 生物技术进展, 2022, 12(1): 17-26.

Figures/Tables 5

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植物	内生固氮菌	部位	参考文献
甘蔗	拜叶林克氏菌（Beijerinck）固氮葡糖醋杆菌（Gluconacetobacterdiazotrophicus）伯克霍尔德氏菌（Burkholderia sp.）微杆菌属（Micrebacterium sp.）变栖克雷伯氏菌（Klebsiella sp.）鞘氨醇单胞菌属（Sphingomonas sp．）苍白杆菌属（Ochrobactrum sp．）微杆菌属（Microbacterium sp．）木糖氧化粪产碱菌（Achromobacterxylosoxidans）嗜麦芽假单胞菌（Stenotrophomonasmaltophilia）根癌土壤杆菌（Agrobacteriumtumefaciens）巴西固氮螺菌（Azospirillumbrasilense）	根、茎、叶、甘蔗汁	[2,10⁃14]
水稻	粪产碱菌（Alcaligenes faccalis）阴沟肠杆菌（Enterobacter cloacae）成团泛菌（Pantoeaagglomerans）肺炎克雷伯氏菌（Klebsiella pneumoniae）无丙二酸柠檬酸杆菌（Citrobacter amalonaticus）	根、茎、叶、种子	[15⁃18]
玉米	肺炎克雷伯氏菌（Kelebesilla pneumomiae）阴沟肠杆菌（Enterobacter cloacae）芽孢杆菌（Bacillus）肠杆菌（Enterobacter）克雷伯氏菌（Klebsiella）假单胞菌（Pseudomona）	根、茎、叶	[19⁃23]
小麦	巴西固氮螺菌属（Azospirillum brasilense）假单胞菌（Pseudomonas）根瘤菌（Rhizobium）芽孢杆菌（Bacillus）黄杆菌（Flavobacterium）	根、茎、叶	[24]
牧草	固氮螺菌（Azospirillum）变栖克雷伯氏菌（Klebsiella variicola）	根、茎、叶	[25⁃26]
蔬菜	假单胞菌属（Pseudomonas）根瘤菌属（Rhizobium）节细菌属（Arthrobacter）类芽孢杆菌属（Paenibacillus）红球菌属（Rhodococcus）黄杆菌属（Flavobacterium）	根、茎、叶	[27]
薯类	越南伯克霍尔德菌（Burkholderia vietnamiensis）短芽孢杆菌属（Brevibacterium）芽孢杆菌属（Bacillus）泛菌属（Pantoea）	根、茎、叶、块茎	[29⁃31]

植物	内生固氮菌	部位	参考文献
甘蔗	拜叶林克氏菌（Beijerinck）固氮葡糖醋杆菌（Gluconacetobacterdiazotrophicus）伯克霍尔德氏菌（Burkholderia sp.）微杆菌属（Micrebacterium sp.）变栖克雷伯氏菌（Klebsiella sp.）鞘氨醇单胞菌属（Sphingomonas sp．）苍白杆菌属（Ochrobactrum sp．）微杆菌属（Microbacterium sp．）木糖氧化粪产碱菌（Achromobacterxylosoxidans）嗜麦芽假单胞菌（Stenotrophomonasmaltophilia）根癌土壤杆菌（Agrobacteriumtumefaciens）巴西固氮螺菌（Azospirillumbrasilense）	根、茎、叶、甘蔗汁	[2,10⁃14]
水稻	粪产碱菌（Alcaligenes faccalis）阴沟肠杆菌（Enterobacter cloacae）成团泛菌（Pantoeaagglomerans）肺炎克雷伯氏菌（Klebsiella pneumoniae）无丙二酸柠檬酸杆菌（Citrobacter amalonaticus）	根、茎、叶、种子	[15⁃18]
玉米	肺炎克雷伯氏菌（Kelebesilla pneumomiae）阴沟肠杆菌（Enterobacter cloacae）芽孢杆菌（Bacillus）肠杆菌（Enterobacter）克雷伯氏菌（Klebsiella）假单胞菌（Pseudomona）	根、茎、叶	[19⁃23]
小麦	巴西固氮螺菌属（Azospirillum brasilense）假单胞菌（Pseudomonas）根瘤菌（Rhizobium）芽孢杆菌（Bacillus）黄杆菌（Flavobacterium）	根、茎、叶	[24]
牧草	固氮螺菌（Azospirillum）变栖克雷伯氏菌（Klebsiella variicola）	根、茎、叶	[25⁃26]
蔬菜	假单胞菌属（Pseudomonas）根瘤菌属（Rhizobium）节细菌属（Arthrobacter）类芽孢杆菌属（Paenibacillus）红球菌属（Rhodococcus）黄杆菌属（Flavobacterium）	根、茎、叶	[27]
薯类	越南伯克霍尔德菌（Burkholderia vietnamiensis）短芽孢杆菌属（Brevibacterium）芽孢杆菌属（Bacillus）泛菌属（Pantoea）	根、茎、叶、块茎	[29⁃31]

内生固氮菌	植物	侵入途径	参考文献
赛鲁普蒂卡草螺菌	水稻	水稻种子的缝隙种脐或者维管系统	[33]
固氮醋酸杆菌	甘蔗	侵入根尖和侧根并形成侵染线样结构	[38]
草螺菌	甘蔗	侧根或2,4⁃D诱导副结节产生的裂缝	[33]
固氮螺菌	牧草	侧根或2,4⁃D诱导副结节产生的裂缝	[33]

内生固氮菌	植物	侵入途径	参考文献
赛鲁普蒂卡草螺菌	水稻	水稻种子的缝隙种脐或者维管系统	[33]
固氮醋酸杆菌	甘蔗	侵入根尖和侧根并形成侵染线样结构	[38]
草螺菌	甘蔗	侧根或2,4⁃D诱导副结节产生的裂缝	[33]
固氮螺菌	牧草	侧根或2,4⁃D诱导副结节产生的裂缝	[33]

Research Progress on Plant Endophytic Nitrogen⁃fixing Bacteria and Their Nitrogen Fixation Mechanism

植物内生固氮菌及其固氮机理研究进展

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