Abstract: With the acceleration of transgenic research and industrialization in China, the importance of transgenic detection has become increasingly prominent. Therefore, the research and development of new methods for rapid and efficient detection has great significance in production and scientific research. In transgenic detection, conventional PCR technology has the characteristics of wide detection range, but it is time-consuming and laborious, and has high requirements for experimental conditions. Meanwhile, colloidal gold protein test paper method is convenient and fast, but the detectable range is narrow. Based on this, a set of method system for rapid transgenic detection based on nucleic acid chromatography was established. After extracting DNA from the samples by liquid ammonia by one-tube method, PCR amplification was carried out directly, and then the PCR amplification products were dripped onto the colloidal gold detection card, and the test results were observed by eyes directly due to the clear difference of color. In this method, corn internal reference genes ZSSⅡB and Zein, soybean internal reference gene SPS, rice internal reference gene Lectin, transgenic element promoter CaMV35S and terminator NOS, insect-resistant genes Cry1Ab/1Ac, herbicide-resistant genes Bar, Pat, CP4-EPSPS and selectable marker genes NPTⅡ and Pmi were finally detected. And the specific transgenic events Mir604 and Bt11 were also successfully detected. The nucleic acid chromatography detection method obtained by the research had the advantages of high sensitivity, efficient, and low requirements for detection conditions. It combined the advantages of PCR and protein test paper detection, and could be widely used in accurate and rapid detection of genetically modified products, providing a good technical support for the safety supervision of genetically modified organisms in China.

Key words: genetically modified maize, nucleic acid chromatography, test strip for rapid transgene detection, qualitative detection

摘要： 随着我国转基因研究及产业化进程提速，转基因检测的重要性日益凸显，因而快速、高效的分子检测新方法的研发具有重要的生产实践及科研意义。在转基因检测中，常规PCR技术具有检测范围广的特点，但较为费时费力，且对实验条件要求较高；而胶体金蛋白试纸法检测方便、快捷，但可检范围窄。基于此，建立了一套基于核酸层析法快速转基因检测的方法体系。将经液氨研磨后的样品通过一管法提取DNA后直接进行PCR扩增，再将PCR扩增产物滴加到胶体金检测卡上，通过直接观察胶体金卡条显色状况进行结果判别。此方法最终检验出玉米内参基因ZSSⅡB及Zein、大豆内参基因SPS、水稻内参基因Lectin，转基因元件启动子CaMV35S及终止子NOS，以及抗虫基因Cry1Ab/1Ac、抗除草剂基因Bar、Pat、CP4-EPSPS及选择标记基因NPTⅡ、Pmi等外源基因；并成功检出特异性转基因事件Mir604及Bt11。研究获得的核酸层析检测方法具有灵敏度高、省时省力且对检测条件要求低等优点，集PCR法与蛋白试纸检测法二者优势于一身，可广泛应用于转基因产品的精确快速检测，为我国转基因生物安全监管提供了良好的技术支撑。

关键词: 转基因玉米, 核酸层析法, 快速转基因检测试纸条, 定性检测