Curr. Biotech. ›› 2016, Vol. 6 ›› Issue (6): 428-434.DOI: 10.3969/j.issn.2095-2341.2016.06.07

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Genetic Stability Analysis of Insect-resistant and Herbicide-tolerance Transgenic Maize HiII-NGc-1

LIU Yang, LIU Xiang-guo, WANG Yang-zhou, ZHAO Fang-fang, ZHANG Yan, XIAO Guo-hong, FENG Shu-dan, HAN Si-ping, YIN Yue-jia   

  1. 1.Jilin Provincial Key Laboratory of Agricultural Biotechnology, Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China;
    2.Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Jilin Gongzhuling 130124, China;
    3.College of Life Science and Technology, Harbin Nomal University, Harbin 150025, China
  • Received:2016-09-26 Online:2016-11-25 Published:2016-11-03

抗虫抗除草剂转基因玉米HiII-NGc-1的遗传稳定性分析

刘洋1,刘相国1,汪洋洲2,赵方方3,张艳1,肖国红3,冯树丹3,韩四平1*,尹悦佳1*   

  1. 1.吉林省农业科学院农业生物技术研究所, 吉林省农业生物技术重点实验室, 长春 130033;
    2.吉林省农业科学院植物保护所, 吉林 公主岭 136100;
    3.哈尔滨师范大学生命科学与技术学院, 哈尔滨 150025
  • 通讯作者: 韩四平，副研究员，研究方向为作物生物技术。E-mail:hansp@jlu.edu.cn。尹悦佳，助理研究员，研究方向为作物生物技术。E-mail:yyjqishi@163.com
  • 作者简介:刘洋，研究实习员，主要从事作物生物技术研究。E-mail:liuyang_20045894@163.com。
  • 基金资助:
    吉林省农业科技创新工程(吉财教［2012］1072);吉林省科技发展计划项目(20160520060JH)资助。

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Abstract: The genetic stability of target genes is the premise for the breeding of transgenic plants. We analyzed the genetic stability of transgenic maize HiII-NGc-1, which approved environmental release in Jilin province, such as integration of the target gene, expression of the target gene, stability of the target traits. cryNGc and bar gene were demonstrated co-integrated into the maize genome DNA at T2~T4 generation by PCR method. RT-PCR and Immunostrip tests demonstrated that CryNGc and PAT proteins were expressed in transgenic maize HiII-NGc-1 at T2~T4 generation. Bioassay for insect resistance and herbicide-tolerance analysis in field showed that transgenic maize HiII-NGc-1 had 5 times glufosinate tolerance, and high resistance against Ostrinia furnacalis at whorl stage and heading stage. Genetic stability of transgenic maize HiII-NGc-1 is the basis for further research of its biological safety evaluation. The results of this study are important for the safety evaluation and industrialization of transgenic insect resistant maize.

Key words: insect-resistant, herbicide-tolerance, transgenic maize, genetic stability

摘要： 目的基因和目标性状遗传稳定性是转基因植物新品种选育和产业化的重要前提。以进入农业部环境释放的抗虫抗除草剂双价转基因玉米HiII-NGc-1为试验材料，采用PCR、RT-PCR和免疫试纸条技术检测转基因玉米连续3代目的基因cryNGc和bar整合及表达的遗传稳定性，采用心叶期除草剂草铵膦抗性鉴定和心叶期/穗期亚洲玉米螟抗性鉴定实验详细分析了目标性状的遗传稳定性。研究结果表明：CryNGc和PAT蛋白在HiII-NGc-1中连续3代遗传稳定，高世代稳定耐受正常5倍大田除草剂草铵膦使用浓度，心叶期和穗期高抗亚洲玉米螟性状稳定，具有潜在的产业化应用价值。转基因玉米HiII-NGc-1目的基因和目标性状遗传稳定性的明确为开展下一步生物安全评价奠定了基础，对复合性状转基因抗虫抗除草剂玉米新品种的选育和产业化具有重要意义。

关键词: 抗虫, 抗除草剂, 转基因玉米, 遗传稳定性

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