Abstract:

As the executor of life activities， the protein function is often reflected in the interaction with other proteins. The study of protein-protein interaction is of great significance for people to understand the prevention of infectious diseases， targeted treatment of multi-gene diseases， and elucidate the molecular mechanism of protein and various complex life phenomena. At present， a variety of techniques have been used to study protein-protein interactions. The difficulty lies in real-time capture of transient or weak protein-protein interactions， mass spectrometry （MS） can solve this difficulty to some extent. Because mass spectrometry can be used to study simple protein complexes and large-scale proteome experiments， the study of protein interactions based on mass spectrometry has been increasingly applied in scientific research. In this paper， the research progress of protein-protein interaction detection methods was reviewed， and the advantages and disadvantages of hydrogen deuterium exchange mass spectrometry and chemical crosslinking mass spectrometry and their applications were emphasized. Finally， the research on protein-protein interaction based on mass spectrometry was summarized and prospected， which aimed to provide reference for further research.

Key words: protein-protein interaction, protein structure, hydrogen deuterium exchange mass spectrometry, chemical cross-linking mass spectrometry

摘要：

蛋白质作为生命活动的执行者，其功能往往体现在与其他蛋白质的相互作用中，研究蛋白-蛋白相互作用对于人们深入了解和预防传染病、靶向治疗多基因疾病、阐明蛋白质的分子作用机制及各种复杂的生命现象具有重要意义。目前，有多种技术被用来研究蛋白间的相互作用，研究难点在于实时捕获瞬时或弱蛋白质间的相互作用，质谱技术（mass spectrometry, MS）可在某种程度上解决该难点。由于质谱技术可研究简单的蛋白质复合物再到大规模的蛋白质组实验，基于质谱技术研究蛋白质间相互作用被越来越多地应用于科学研究中。综述了蛋白质间相互作用检测方法的研究进展，重点介绍了氢氘交换质谱法和化学交联质谱法研究蛋白质间相互作用的优缺点及其应用，最后对基于质谱技术研究蛋白质间相互作用进行了总结与展望，以期为深入开展相关研究提供借鉴。

关键词: 蛋白-蛋白相互作用, 蛋白质结构, 氢氘交换质谱法, 化学交联质谱法