Abstract: Chondroitin sulfate (CS) is a kind of protein viscose polysaccharide extracted from animal tissues, which plays a role in immunomodulation, relieving joint pain, anticoagulant activity and protecting neurons. It is of great significance to develop CS sensor for the coarse screening of the relevant pharmaceutical and health care products in the biomedical industry. In this paper, based on the characteristics of simple preparation, rapid response and strong selectivity of fluorescent sensors, a cationic polymer PDDA was used to induce the self-assembly of the pyrene-based molecule HPTS, resulting in fluorescence quenching to construct the sensor. The addition of the analyte caused the recovery of the fluorescence intensity at 510 nm. We finally established a rapid analysis method for CS in the actual sample. The method showed a good linear relationship at a concentration of 0～5 μmol·L-1. The linear equation was y=1.907 52+0.273 59x (R2=0.998 2), the detection limit was 2.8 μmol·L-1, and the sample recovery was 104%～121%. This sensor features good water solubility, strong specificity and intuitive visualization.

Key words: chondroitin sulfate, pyrene, rapid detection, fluorescence quenching

摘要： 硫酸软骨素（chondroitin sulfate，CS）是一类从动物组织中提取的蛋白类黏性多聚糖，在人体免疫调节、缓解关节疼痛、抗凝活性和保护神经元等方面发挥效用，开发硫酸软骨素传感器对生物医药行业研发相关药剂、保健产品质量的粗筛具有重要意义。利用荧光传感器制备简便、响应迅速、选择性强的特点，用阳离子聚合物PDDA诱导芘基分子HPTS自组装，导致荧光淬灭构建传感器。研究结果显示，待测物的加入引起510 nm处荧光强度的恢复，最终建立了实际样品中CS的快速分析方法。构建的硫酸软骨素荧光传感器在浓度0～5 μmol·L-1间呈现良好的线性关系，线性方程为y=1.907 52+0.273 59x(R2=0.998 2)，检出限为2.8 μmol·L-1，样品加标回收率在104%～121%之间。该传感器具有水溶性好、特异性强、直观可视等特点。

关键词: 硫酸软骨素, 芘, 快速检测, 荧光传感器