GhCPK4蛋白生物信息学分析及其在棉花中的表达模式

doi:10.19586/j.2095-2341.2025.0168

生物技术进展 ›› 2026, Vol. 16 ›› Issue (3): 638-646.DOI: 10.19586/j.2095-2341.2025.0168

GhCPK4蛋白生物信息学分析及其在棉花中的表达模式

丁璇¹^,²(), 张祖英¹^,², 玛依拉·玉素音², 吴佳佳¹^,², 苏秀娟¹(), 胡文冉²()

^1.新疆农业大学农学院，乌鲁木齐 830052
^2.新疆维吾尔自治区农业科学院生物育种实验室，乌鲁木齐 830091

收稿日期:2025-11-24 接受日期:2026-02-12 出版日期:2026-05-25 发布日期:2026-07-14
通讯作者: 苏秀娟,胡文冉
作者简介:丁璇 E-mail: dx_0201@163.com
基金资助:
国家自然科学基金地区基金项目(32360510)

Bioinformatics Analysis of GhCPK4 Protein and its Expression Pattern in Cotton

Xuan DING¹^,²(), Zuying ZHANG¹^,², MAYILA·Yusuyin², Jiajia WU¹^,², Xiujuan SU¹(), Wenran HU²()

^1.Agricultural College，Xinjiang Agricultural University，Urumqi 830052，China
^2.Biological Breeding Laboratory，Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences，Urumqi 830091，China

Received:2025-11-24 Accepted:2026-02-12 Online:2026-05-25 Published:2026-07-14
Contact: Xiujuan SU,Wenran HU

摘要/Abstract

摘要：

大量研究表明，钙依赖蛋白激酶CPKs在植物响应逆境胁迫过程中发挥着重要作用。为深入探究GhCPK4基因的作用机制，对GhCPK4蛋白进行生物信息学分析，并研究其在棉花中的表达模式。结果表明，GhCPK4蛋白含有蛋白激酶结构域和EF手型结构域，序列保守性较高。GhCPK4无信号肽，主要定位于细胞核中，属于不稳定的亲水性蛋白；具有62个磷酸化和6个糖基化修饰位点；其二级结构主要由无规则卷曲和α-螺旋构成，存在形式为单体。GhCPK4基因在棉花所有组织中均有表达，但表达水平存在显著差异，花瓣中的表达高于其他组织。研究结果可为棉花GhCPK4基因在棉花黄萎病中生物学功能的深入研究提供理论依据。

关键词: 棉花, GhCPK4, 生物信息学, 表达模式

Abstract:

A large number of studies have shown that calcium-dependent protein kinases （CPKs） play important roles in plant responses to abiotic and biotic stresses. To further elucidate the mechanism underlying GhCPK4 gene function， bioinformatics analyses and expression pattern profiling of the GhCPK4 protein were conducted. The results showed that the GhCPK4 protein contains a protein kinase domain and EF-hand domains， with high sequence conservation. GhTCP4 lacks a signal peptide， is mainly localized in the nucleus and is characterized as an unstable hydrophilic protein. It has 62 phosphorylation and 6 glycosylation modification sites. Its secondary structure is mainly composed of random coils and α-helices， and its existence form is monomer. The GhCPK4 gene was expressed in all cotton tissues examined， however， the expression levels varied significantly， with the highest leves observed in petals compared to other tissues. This study provides a theoretical foundation for further research into the biological function of the GhCPK4 gene in cotton resistance to Verticillium wilt.

Key words: cotton, GhCPK4, bioinformatics, expression pattern

中图分类号:

Q819

丁璇, 张祖英, 玛依拉·玉素音, 吴佳佳, 苏秀娟, 胡文冉. GhCPK4蛋白生物信息学分析及其在棉花中的表达模式[J]. 生物技术进展, 2026, 16(3): 638-646.

Xuan DING, Zuying ZHANG, MAYILA·Yusuyin, Jiajia WU, Xiujuan SU, Wenran HU. Bioinformatics Analysis of GhCPK4 Protein and its Expression Pattern in Cotton[J]. Current Biotechnology, 2026, 16(3): 638-646.

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https://swjsjz.magtechjournal.com/CN/Y2026/V16/I3/638

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GhCPK4蛋白生物信息学分析及其在棉花中的表达模式

Bioinformatics Analysis of GhCPK4 Protein and its Expression Pattern in Cotton

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