人源化斑马鱼模型的构建技术创新及其在疾病研究中的应用进展

doi:10.19586/j.2095-2341.2026.0019

生物技术进展 ›› 2026, Vol. 16 ›› Issue (3): 618-625.DOI: 10.19586/j.2095-2341.2026.0019

人源化斑马鱼模型的构建技术创新及其在疾病研究中的应用进展

孙晓月(), 朱然然, 刘可春, 蹇雪慧, 王雪, 张云, 夏青, 于元帅, 侯海荣, 孙晨()

齐鲁工业大学（山东省科学院）生物研究所，山东省人类疾病斑马鱼模型与药物筛选工程技术研究中心，济南 250103

收稿日期:2026-01-28 接受日期:2026-02-27 出版日期:2026-05-25 发布日期:2026-07-14
通讯作者: 孙晨
作者简介:孙晓月 E-mail: 13854398191@163.com；
基金资助:
齐鲁工业大学（山东省科学院）科教产融合试点工程重大创新类项目(2025zDzx14)

Construction Technology Innovation and Application Progress in the Disease of Humanized Zebrafish Model

Xiaoyue SUN(), Ranran ZHU, Kechun LIU, Xuehui JIAN, Xue WANG, Yun ZHANG, Qing XIA, Yuanshuai YU, Hairong HOU, Chen SUN()

Engineering Research Center of Zebrafish Models for Human Diseases and Drug Screening of Shandong Province，Biology Institute，Qilu University of Technology （Shandong Academy of Sciences），Jinan 250103，China

Received:2026-01-28 Accepted:2026-02-27 Online:2026-05-25 Published:2026-07-14
Contact: Chen SUN

摘要/Abstract

摘要：

人源化斑马鱼模型可精准模拟人类生理和病理过程，有效突破物种间的研究壁垒，现已成为生物医药研究领域的重要载体。基于此，系统阐述了斑马鱼在构建人源化动物模型中的天然优势，对成簇规律间隔短回文重复序列/CRISPR相关蛋白9（clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9，CRISPR/Cas9）、Tol2转座子和异种移植3种常用人源化斑马鱼模型构建技术的技术原理、特点和优势等进行了较为全面的总结，并重点介绍了人源化斑马鱼模型在心血管、肿瘤、神经系统和肝脏等多个疾病领域的应用现状和突破，提出了其在未来研究中可能的发展方向，如多模型综合应用、多技术融合创新等，以期为人源化斑马鱼模型的研究提供一定的技术参考，进一步推动医药研发领域的研究。

关键词: 人源化斑马鱼模型, 基因编辑, 转座子, 异种移植

Abstract:

Humanized zebrafish models can accurately simulate human physiological and pathological processes， effectively overcome interspecies research barriers， and have become important research carriers in biomedicine. On this basis， the paper systematically elaborated on the inherent advantages of zebrafish for constructing humanized animal models， and comprehensively summarized the technical principles， characteristics and strengths of three commonly used approaches for establishing humanized zebrafish models， namely clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9（CRISPR/Cas9）， Tol2 transposon system， and xenotransplantation. It further highlighted the research progress and latest breakthroughs of humanized zebrafish models in multiple disease areas， including cardiovascular diseases， tumors， neurological disorders and liver diseases. Potential future research prospects were also proposed， including the combined application of diverse models and the integrated innovation of multiple technologies. The review aims to provide technical references for researchs in humanized zebrafish models and further advance studies on pharmaceutial research and development.

Key words: humanized zebrafish models, gene editing, transposon, xenotransplantation

中图分类号:

Q95.33

孙晓月, 朱然然, 刘可春, 蹇雪慧, 王雪, 张云, 夏青, 于元帅, 侯海荣, 孙晨. 人源化斑马鱼模型的构建技术创新及其在疾病研究中的应用进展[J]. 生物技术进展, 2026, 16(3): 618-625.

Xiaoyue SUN, Ranran ZHU, Kechun LIU, Xuehui JIAN, Xue WANG, Yun ZHANG, Qing XIA, Yuanshuai YU, Hairong HOU, Chen SUN. Construction Technology Innovation and Application Progress in the Disease of Humanized Zebrafish Model[J]. Current Biotechnology, 2026, 16(3): 618-625.

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链接本文: https://swjsjz.magtechjournal.com/CN/10.19586/j.2095-2341.2026.0019

https://swjsjz.magtechjournal.com/CN/Y2026/V16/I3/618

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人源化斑马鱼模型的构建技术创新及其在疾病研究中的应用进展

Construction Technology Innovation and Application Progress in the Disease of Humanized Zebrafish Model

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