小麦TaWRKY13转录因子参与ABA介导叶片衰老的功能研究

doi:10.19586/j.2095-2341.2025.0085

生物技术进展 ›› 2025, Vol. 15 ›› Issue (6): 1020-1030.DOI: 10.19586/j.2095-2341.2025.0085

• 研究论文 • 上一篇

小麦TaWRKY13转录因子参与ABA介导叶片衰老的功能研究

乔华亮¹^,²(), 王娇¹, 焦博¹, 柳洪², 李俊明², 王耕², 周春江², 周硕¹()

^1.河北省农林科学院生物技术与食品科学研究所，河北省植物基因工程重点实验室，石家庄 050051
^2.河北师范大学生命科学学院，石家庄 050024

收稿日期:2025-07-28 接受日期:2025-10-22 出版日期:2025-11-25 发布日期:2026-01-04
通讯作者: 周硕
作者简介:乔华亮E-mail: qiaohl1006@163.com；
基金资助:
河北省自然科学基金项目(C2022301072);农业生物育种国家科技重大专项(2023ED040230303);河北省农林科学院现代农业科技创新专项(2022KJCXZX-SSS-5)

Functional Analysis of the Wheat TaWRKY13 Transcription Factor in ABA-mediated Leaf Senescence

Hualiang QIAO¹^,²(), Jiao WANG¹, Bo JIAO¹, Hong LIU², Junming LI², Geng WANG², Chunjiang ZHOU², Shuo ZHOU¹()

^1.Key Laboratory of Plant Gene Engineering of Hebei Province，Institute of Biotechnology and Food Science，Hebei Academy of Agriculture and Forestry Sciences，Shijiazhuang 050051，China
^2.College of Life Sciences，Hebei Normal University，Shijiazhuang 050024，China

Received:2025-07-28 Accepted:2025-10-22 Online:2025-11-25 Published:2026-01-04
Contact: Shuo ZHOU

摘要/Abstract

摘要：

小麦TaWRKY13转录因子是调控叶片衰老的重要基因，对其作用机制进行解析可影响农作物的产量和品质。利用农杆菌转化法获得了TaWRKY13的过表达小麦，并观察到TaWRKY13能够促进叶片衰老。通过外源脱落酸（abscisic acid，ABA）处理，发现TaWRKY13过表达株系能够响应ABA的处理，并可加速TaWRKY13过表达衰老进程。通过实时定量PCR、ChIP-PCR和凝胶迁移（electrophoretic mobility shift assay，EMSA）实验证明TaWRKY13能够通过直接结合TaNCED5影响ABA的变化，进而改变叶片衰老进程。综上，TaWRKY13直接结合TaNCED5调控ABA的合成，影响ABA的含量，进而影响叶片衰老的进程。结果可为阐明小麦中叶片的衰老机制提供理论依据。

关键词: 叶片衰老, WRKY转录因子, 脱落酸

Abstract:

Transcription factor TaWRKY13 of wheat is a key gene regulating leaf senescence， and analyzing its mechanism of action can affect crop yield and quality. Using agrobacterium-mediated transformation， we obtained wheat overexpressing TaWRKY13 and observed that TaWRKY13 promotes leaf senescence. Exogenous abscisic acid （ABA） treatment revealed that TaWRKY13 responds to ABA and accelerates the senescence process in TaWRKY13-overexpressing plants. Real-time quantitative PCR， ChIP-PCR， and electrophoretic mobility shift assay （EMSA） results demonstrated that TaWRKY13 influences ABA dynamics by directly binding to TaNCED5， thereby altering the leaf senescence process. In summary， TaWRKY13 directly binds to TaNCED5 to regulate ABA synthesis， affecting ABA content and thus influencing the process of leaf senescence. The results can provide a theoretical basis for elucidating the aging mechanism of leaves in wheat.

Key words: leaf senescence, WRKY transcription factor, abscisic acid

中图分类号:

乔华亮, 王娇, 焦博, 柳洪, 李俊明, 王耕, 周春江, 周硕. 小麦TaWRKY13转录因子参与ABA介导叶片衰老的功能研究[J]. 生物技术进展, 2025, 15(6): 1020-1030.

Hualiang QIAO, Jiao WANG, Bo JIAO, Hong LIU, Junming LI, Geng WANG, Chunjiang ZHOU, Shuo ZHOU. Functional Analysis of the Wheat TaWRKY13 Transcription Factor in ABA-mediated Leaf Senescence[J]. Current Biotechnology, 2025, 15(6): 1020-1030.

图/表 8

表1 本文所涉及的载体

Table 1 The carrier involved in this article

用途	载体	细菌内抗生素	植物内抗生菌
原核表达载体	pMal-C2X	氨苄（ampicillin, Amp）	利福平（rifampicin, Rfp）
过表达植物载体	pTCK303	卡那（kanamycin, Kan）	潮霉素（hygromycin, Hyg）

图1 TaWRKY13过表达载体及在烟草中表达验证注：WT—野生对照组；OE—瞬时表达TaWRKY-13-Flag烟草。

Fig. 1 TaWRKY13 overexpression vector and expression validation in tobacco

图2 TaWRKY13过表达小麦表型观察A：TaWRKY13转基因小麦表型；B：叶绿素含量；C：离子渗漏率；D：蛋白表达量；E~F:叶片衰老maker基因（TaSAG3、TaSAG5）表达量。**表示与WT相比在P0.01水平上有统计学差异。

Fig. 2 Observation of phenotype of TaWRKY13 overexpression in wheat

图3 TaWRKY13过表达小麦叶片在黑暗处理下表型A：黑暗处理下过表达小麦叶片表型；B：TaWRKY13过表达小麦转录水平表达检测；C：叶绿素含量；D：离子渗漏率。**表示与WT相比在P0.01水平上有统计学差异。

Fig. 3 Phenotype of wheat leaves overexpressing TaWRKY13 under dark treatment

图4 TaWRKY13过表达小麦的籽粒性状注：*，**分别表示与WT组相比在P0.05和P0.01水平上有统计学差异。

Fig. 4 Grain traits of TaWRKY13 overexpression wheat

图5 ABA处理下TaWRKY13过表达小麦叶片的表型注：**表示与WT组相比在P0.01水平上有统计学差异。

Fig. 5 Phenotype of TaWRKY13 overexpression in wheat leaves under ABA treatment

图6 TaWRKY13过表达小麦中ABA合成和信号通路相关基因检测注：*，**分别表示与WT组相比在P0.05和P0.01水平上有统计学差异。

Fig. 6 Detection of ABA synthesis and signaling pathway-related genes in wheat overexpressing TaWRKY13

图7 TaWRKY13与TaNCED5启动子的相互作用A：TaWRKY13与TaNCED5启动子的ChIP-PCR；B：TaWRKY13与TaNCED5启动子的凝胶迁移

Fig. 7 The interaction between TaWRKY13 and promoter of TaNCED5

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小麦TaWRKY13转录因子参与ABA介导叶片衰老的功能研究

Functional Analysis of the Wheat TaWRKY13 Transcription Factor in ABA-mediated Leaf Senescence

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