生物技术进展 ›› 2024, Vol. 14 ›› Issue (2): 173-181.DOI: 10.19586/j.2095-2341.2023.0157
• 进展评述 • 下一篇
孙佳琪1,2(), 郭嘉2, 张闯2, 柳青2, 王梓钰2, 夏涵超2, 钱步轩2, 赵方方2, 王棋2, 刘剑锋1(
), 刘相国2(
)
收稿日期:
2023-12-07
接受日期:
2024-01-04
出版日期:
2024-03-25
发布日期:
2024-04-17
通讯作者:
刘剑锋,刘相国
作者简介:
孙佳琪E-mail: 1094233375@qq.com
基金资助:
Jiaqi SUN1,2(), Jia GUO2, Chuang ZHANG2, Qing LIU2, Ziyu WANG2, Hanchao XIA2, Buxuan QIAN2, Fangfang ZHAO2, Qi WANG2, Jianfeng LIU1(
), Xiangguo LIU2(
)
Received:
2023-12-07
Accepted:
2024-01-04
Online:
2024-03-25
Published:
2024-04-17
Contact:
Jianfeng LIU,Xiangguo LIU
摘要:
近年来,农业生产面临磷源匮乏和环境污染的双重挑战,亚磷酸脱氢酶(phosphite dehydrogenase, PTDH)的发现和应用为解决这一问题提供了新的思路。在微生物中,PTDH使得非无菌环境下的高效发酵成为可能,降低了成本并提高了生产效率。在植物中,PTDH不仅解决了磷源问题,还为除草剂的开发提供了新的途径。此外,亚磷酸盐可作为一种潜在的除草剂和磷源使用,相比传统除草剂更环保、成本更低、稳定性更好。尽管亚磷酸脱氢酶的应用研究取得了显著进展,但目前仍处于功能解析和概念验证阶段,对亚磷酸脱氢酶的催化机理、蛋白结构和酶活改良等方面的研究还需进一步深入。综述了PTDH在微生物、模式植物(如拟南芥和烟草)、经济作物(如棉花和油菜)以及粮食作物(如水稻和玉米)中的应用进展。随着蛋白质定向进化技术、基因转化和基因编辑技术的发展,未来将进一步优化亚磷酸脱氢酶的应用效果,为作物的高效利用亚磷酸盐和环境保护提供更多可能。
中图分类号:
孙佳琪, 郭嘉, 张闯, 柳青, 王梓钰, 夏涵超, 钱步轩, 赵方方, 王棋, 刘剑锋, 刘相国. 亚磷酸脱氢酶在基因工程改造微生物和植物中的研究进展[J]. 生物技术进展, 2024, 14(2): 173-181.
Jiaqi SUN, Jia GUO, Chuang ZHANG, Qing LIU, Ziyu WANG, Hanchao XIA, Buxuan QIAN, Fangfang ZHAO, Qi WANG, Jianfeng LIU, Xiangguo LIU. Research Progress of Phosphite Dehydrogenase in Genetically Engineered Microorganisms and Plants[J]. Current Biotechnology, 2024, 14(2): 173-181.
基因来源 | 微生物种类 | 目标性状及产业化效果 | 参考文献 |
---|---|---|---|
施氏假单胞菌 (Pseudomonas stutzeri) | 枯草芽孢杆菌 (Bacillus subtilis) | 建立抗菌污染体系,提高产物生产效率 | [ |
施氏假单胞菌 (Pseudomonas stutzeri) | 谷氨酸棒状杆菌 (Corynebacterium glutamicum) | 缩短发酵周期、简化发酵过程,降低生产成本,但目前连续发酵的赖氨酸产量不是很理想 | [ |
施氏假单胞菌WM88 (Pseudomonas sp. WM88) | 莱恩衣藻 (Chlamydomonas reinhardtii) | 作为选择标记基因,建立转质体系使其成为鉴定转化菌株的有效工具 | [ |
表1 亚磷酸脱氢酶基因在微生物中的应用
Table 1 Application of phosphite dehydrogenase genes in microorganisms
基因来源 | 微生物种类 | 目标性状及产业化效果 | 参考文献 |
---|---|---|---|
施氏假单胞菌 (Pseudomonas stutzeri) | 枯草芽孢杆菌 (Bacillus subtilis) | 建立抗菌污染体系,提高产物生产效率 | [ |
施氏假单胞菌 (Pseudomonas stutzeri) | 谷氨酸棒状杆菌 (Corynebacterium glutamicum) | 缩短发酵周期、简化发酵过程,降低生产成本,但目前连续发酵的赖氨酸产量不是很理想 | [ |
施氏假单胞菌WM88 (Pseudomonas sp. WM88) | 莱恩衣藻 (Chlamydomonas reinhardtii) | 作为选择标记基因,建立转质体系使其成为鉴定转化菌株的有效工具 | [ |
基因来源 | 植物种类 | 目标性状及意义 | 参考文献 |
---|---|---|---|
施氏假单胞菌WM88 (Pseudomonas sp. WM88) | 拟南芥 (Arabidopsis thaliana) | 转基因模式植物能代谢亚磷酸盐,为后续开发亚磷酸盐作为除草剂提供基础 | [ |
施氏假单胞菌WM88 | 烟草 (Nicotiana tabacum) | 转基因模式植物代谢亚磷酸盐,将亚磷酸盐作为新的磷源 | [ |
施氏假单胞菌WM88 | 棉花 (Gossypium spp.) | 建立ptxD/Phi筛选体系,作为除草剂 | [ |
施氏假单胞菌WM88 | 油菜 (Brassica napus) | 使其代谢亚磷酸盐,为后续油菜利用亚磷酸盐作为磷肥奠定基础 | [ |
罗尔斯通菌 4506 (Ralstonia 4506) | 水稻 (Oryza sativa) | 作为筛选标记基因,增加了筛选效率,而且降低了对自然的安全隐患 | [ |
施氏假单胞菌WM88 | 玉米 (Zea mays) | 建立ptxD/Phi选择标记系统,虽然转基因玉米能代谢亚磷酸盐,但愈伤组织的转化频率不高,后续需要对例如亚磷酸盐浓度等进行改进 | [ |
表2 亚磷酸脱氢酶基因在植物中的应用
Table 2 Application of phosphite dehydrogenase genes in plants
基因来源 | 植物种类 | 目标性状及意义 | 参考文献 |
---|---|---|---|
施氏假单胞菌WM88 (Pseudomonas sp. WM88) | 拟南芥 (Arabidopsis thaliana) | 转基因模式植物能代谢亚磷酸盐,为后续开发亚磷酸盐作为除草剂提供基础 | [ |
施氏假单胞菌WM88 | 烟草 (Nicotiana tabacum) | 转基因模式植物代谢亚磷酸盐,将亚磷酸盐作为新的磷源 | [ |
施氏假单胞菌WM88 | 棉花 (Gossypium spp.) | 建立ptxD/Phi筛选体系,作为除草剂 | [ |
施氏假单胞菌WM88 | 油菜 (Brassica napus) | 使其代谢亚磷酸盐,为后续油菜利用亚磷酸盐作为磷肥奠定基础 | [ |
罗尔斯通菌 4506 (Ralstonia 4506) | 水稻 (Oryza sativa) | 作为筛选标记基因,增加了筛选效率,而且降低了对自然的安全隐患 | [ |
施氏假单胞菌WM88 | 玉米 (Zea mays) | 建立ptxD/Phi选择标记系统,虽然转基因玉米能代谢亚磷酸盐,但愈伤组织的转化频率不高,后续需要对例如亚磷酸盐浓度等进行改进 | [ |
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