关键词: 疫苗, 分离纯化, 层析, 膜分离

Abstract: With the development of the vaccine industry in our country and peoples deepening understanding of vaccine safety and side effects, extracted vaccine cannot satisfied the demand, therefore it is of vital importance to achieve high-purified vaccine. The review summed up the development of vaccine purification at present and its trend at home and abroad. Centrifugation and precipitation, as traditional methods, were widely used in vaccine production. Membrane and chromatography played a significant role in purification. Some novel methods, for instance, monolith, membrane chromatography and simulated moving bed, were highlighted and the work for future was discussed.

Key words: vaccine, purification, chromatography, membrane separation