关键词: 非生物逆境, 生育酚, 抗氧化, 玉米, 抗氧化酶

Abstract: Tocopherols are a group of powerful antioxidants, α-tocopherol is the most effective component. In this study, transgenic GmTMT2a plants (TP) with enriched-α-tocopherol and wild type plants (WT) were analyzed under drought stress. Results showed that H2O2 was accumulated in both TP plants and WT plants, however H2O2 content was less in TP plants than that in WT plants. We further analyzed the activity of antioxidant enzymes SOD, POD and CAT, and results showed that CAT actiity was obviously enhanced in TP plants compared with WT plants. Moreover P5CS and TPS transcripts were upregulated more in TP plants than WT. Our data showed that GmTMT2a increased drought tolerance of transgenic maize through promoting CAT, TPS and P5CS expression.

Key words: abiotic stress, tocopherols, antioxidant, maize, antioxidant enzymes