关键词: 聚酮合酶, 真菌, 苯二酚内酯, 生物合成

Abstract: Fungal benzenediol lactones, biosynthesized by a pair of highly reduced polyketide synthases and non-reduced polyketide synthases, have diverse structures and varied biological activities. Fungal polyketide synthases contain multiple functional domains which catalyze the specific structure of final products. We found that unnatural polyketides could be synthesized in the yeast Saccharomyces cerevisiae co-expressing different reduced fungal polyketide synthases and non-reduced polyketide synthases with functional domain swaps. Furthermore, identification pattern of acyltransferase domain was studied and discussed.

Key words: polyketide synthases, fungi, benzenediol lactones, biosynthesis