Abstract: In this study, thirty-two varieties of the middle late maturing group and machine transplanting group district experiments in Shandong Province as well as sixteen cultivars of rice breeding consortium district experiments of Lianyungang Agricultural Academy of Agricultural Sciences were detected with the functional markers of rice blast resistance genes Pi-ta, Pi-b, Pi54 and Pikm. Based on genotype and rice blast resistance identifications, correlation analysis between genotype and phenotype was conducted. Results showed fifteen varieties containing rice blast resistance gene Pi-ta, twenty-five varieties containing Pi-b, twenty-six varieties containing Pi54, and twenty-one varieties containing Pikm in fourty-eight varieties. Luzidao 7 and Lianjing 14ZD24 contain four resistance alleles. Thirteen varieties, including YS-6-6, Jidao 1 and D400, etc, contain three resistance alleles. Eight varieties, including Lindao 10, Feng dao 2, Tianhenuo 303, etc, have no resistance alleles. The results of rice blast identification indicated that four varieties,including Jidao 1,Shengdao 072, Lianjing 14JD24 showed moderate resistance (MR). Twenty-six varieties, including Lindao10, YS-6-6, Shengdao 053 showed moderate susceptibility (MS). Fifteen varieties, including Jindao180, Lin 13-105, Shengdao 504 showed susceptibility (S).H11-15,Runnong9, Jindao160 showed high susceptibility (HS). Pi-ta, Pi-b, Pi54 and Pikm, have been widely used in rice blast resistance breeding in Huang-huai region. Correlation analysis showed that the rice blast resistance gene Pikm was significantly correlated with blast resistance overall index (r=0.477 5，P<0.01）.

Key words: rice blast, Pi-ta, Pi-b, Pi54, Pikm, molecular marker detection

摘要： 利用水稻稻瘟病抗病基因Pi-ta、Pi-b、Pi54 和Pikm的功能标记对2016年山东省水稻中晚熟组区试、机插秧组区试的32个参试品系及连云港农业科学院科企水稻联合体黄淮粳稻区试16个品系进行了分子标记检测，结合稻瘟病抗性接种鉴定，对基因型与表型进行相关性分析。结果表明48个品种中携带Pi-ta、Pi-b、Pi54和Pikm抗性基因的品种数分别为15个、25个、26个和21个，其中鲁资稻7号、连粳14JD24含有4个基因的抗性等位基因，YS-6-6、济稻1号、D400等13个品种分别含有3个基因的抗性等位基因，临稻10号、丰稻2号、天和糯303等8个品种不含抗性等位基因。稻瘟病鉴定结果表明，48份品种中，济稻1号、圣稻072、连粳14JD24等4个品种表现中抗（MR）；临稻10、YS-6-6、圣稻053等26个品种表现中感（MS）；晶稻180、临13-105、圣稻504等15个品种表现感病（S）；H11-15、润农9号、晶稻160表现高感（HS）。Pi-ta、Pi-b、Pi54、Pikm 4个抗性基因已在黄淮区粳稻抗稻瘟病育种中得到广泛应用。其中Pikm与稻瘟病抗性综合指数存在显著相关性（r=0.477 5，P<0.01）。

关键词: 稻瘟病, Pi-ta, Pi-b, Pi54, Pikm, 分子标记检测