journal1 ›› 2011, Vol. 1 ›› Issue (3): 184-188.DOI: 10.3969/j.issn.2095-2341.2011.03.06

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Research Progress on Sortase and its Application in Biotechnology

JIANG Li-bin, WEN Ying, CHEN Zhi   

  1. State Key Laboratories for Agro-Biotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, China
  • Received:2011-05-11 Online:2011-09-25 Published:2011-06-02

分选酶的研究进展及其在生物技术中的应用

姜利滨,文莹,陈芝*   

  1. 中国农业大学农业生物技术国家重点实验室, 北京 100193
  • 通讯作者: 陈芝,副教授,博士,主要从事链霉菌遗传学研究。Tel:010-62732715; E-mail:chenzhi@cau.edu.cn
  • 作者简介:姜利滨,硕士研究生,主要从事链霉菌遗传学研究。
  • 基金资助:

    农业部微生物资源与利用开放重点实验室青年基金项目(201002)资助。

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Abstract: Sortases are membrane-bound transpeptidases produced by Gram-positive bacteria to anchor cell surface proteins covalently to peptidoglycan in the cell wall. Due to its specific reaction, sortases are exploited for a range of biotechnology application, such as Gram-positive bacterial cell-surface display and protein engineering, etc. This paper reviews research progress on sortase and its application in biotechnology.

Key words: sortase, surface protein, surface display, protein linkage

摘要： 分选酶(sortase)普遍存在于革兰氏阳性细菌中,是一类膜结合的转肽酶,负责将表面蛋白共价结合到细胞壁的肽聚糖上。由于其独特的作用机制,分选酶在生物技术领域具有广阔的应用前景,可应用于革兰氏阳性菌的表面展示、蛋白质工程等。

关键词: 分选酶, 表面蛋白, 表面展示, 蛋白质连接

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