Abstract: To minimize environmental contamination and waste of resource, distillers’ grains (DG) from Chinese liquor industry was used to produce reducing sugars for Bacillus thuringiensis (Bt) cultivation. Ca(OH)2 and NaOH were used to pretreat DG at different concentrations and temperatures. HPLC was used to determine the yield of reducing sugars, followed by cultivating Bt using distillers’ grains extract. The results showed that the highest yield of reducing sugars were obtained by  0.75% NaOH for 1 h at 100℃ (81 g/kg). However, the highest spore count was obtained by 1% Ca(OH)2 for 1 h at 50℃, and it was as high as 4.6×107 CFU/mL. DG methods could not only reduce industrial waste and environmental pollution, but also  promote Bt industrialization.

Key words: alkali pretreatment, distillers&rsquo, grains, biomass, Bacillus thuringiensis

摘要： 为了减少环境污染和资源浪费，利用浓香型白酒丢糟作为原料生产还原糖用于培养苏云菌芽胞杆菌(Bt)。采用不同浓度、温度、氢氧化钙和氢氧化钠前处理浓香型白酒丢糟，HPLC测定前处理液还原糖产量，并利用前处理液培养Bt。结果表明，用0.75% NaOH在100℃下处理1 h后，还原糖产量最高（81 g/kg）。而芽胞产量最高的前处理条件为1% Ca(OH)2在50℃下处理1 h，芽胞产量高达4.6×107 CFU/mL。利用白酒丢糟前处理液培养Bt，既能有效的减少资源浪费和环境污染，又能实现Bt制剂商业化。

关键词: 碱前处理, 白酒丢糟, 生物质, 苏云金芽胞杆菌