Curr. Biotech. ›› 2016, Vol. 6 ›› Issue (5): 341-345.DOI: 10.3969/j.issn.2095-2341.2016.05.06

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The TLC Identification and the Cyclovirobuxine D Content Determination in Different Parts of Buxus sinica from Guizhou

YANG Juan-yan, WANG Fang, GUO Ting4, WU Lin-lin4, HUANG Hong-mei, XU Qian-li, MAO Xiang-jun   

  1. 1.Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;
    2.Guizhou Provincial of Food and Drug Inspection Institute, Guiyang 550004, China;
    3.Guizhou Zunyi Medical College, GuiZhou Zunyi 563003, China;
    4.Guizhou Medical University, Guiyang 550004, China
  • Received:2016-05-27 Online:2016-09-25 Published:2016-07-06

黔产小叶黄杨中环维黄杨星D的鉴别及不同部位含量测定

杨娟艳1,2,王芳3,郭婷4,吴琳琳4,黄红梅1,许乾丽2,茅向军2*   

  1. 1.贵阳中医学院, 贵阳 550002;
    2.贵州省食品药品检验所, 贵阳 550004;
    3.遵义医学院, 贵州 遵义 563003;
    4.贵州医科大学, 贵阳 550004
  • 通讯作者: 茅向军，主任药师，研究方向为药品质量研究。Tel:0851-86808857；E-mail:1074459931@qq.com
  • 作者简介:杨娟艳，硕士研究生，研究方向为中药及民族药质量控制与新药研究。E-mail:1274191418@qq.com。
  • 基金资助:
    贵州省科学技术基金（黔科合J字\[2013\]2032号）资助。

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Abstract: The paper studied the cyclovirobuxine D content of Guizhou Buxus sinica with thin-layer chromatography(TLC) profile,  and compared the cyclovirobuxine D content in different parts of Guizhou Buxus sinica, which was expected to provide scientific basis for the development of Buxus sinica resources. The TLC was used to study cyclovirobuxine D profile in Buxus sinica; a HPLC-CAD method was used to measure cyclovirobuxine D content in different parts of Buxus sinica. Results showed that cyclovirobuxine D spots were clear in the TLC; the main stem had the largest cyclovirobuxine D content but the lowest content in leaves. These methods could be used in thin-layer identification and content determination of the Buxus sinica. In order to reasonable develop and utilize Guizhou Buxus sinica, the best medicinal part is coarse stem.

Key words: Buxus sinica, TLC, different parts, cyclovirobuxine D

摘要： 对黔产小叶黄杨中环维黄杨星D进行薄层色谱研究，并比较了小叶黄杨不同部位环维黄杨星D的含量，为合理开发小叶黄杨的药用资源提供科学依据。采用薄层色谱法对小叶黄杨中环维黄杨星D进行薄层色谱研究，采用高效液相色谱电喷雾检测器法对小叶黄杨不同部位环维黄杨星D进行含量测定。结果显示：薄层色谱中环维黄杨星D斑点清晰；高效液相色谱中小叶黄杨不同部位环维黄杨星D的含量差异很大，粗茎含量最高，叶子含量最低。该方法可用于小叶黄杨的薄层鉴别和含量测定，为了合理开发和利用黔产小叶黄杨，其药用部位以粗茎最好。

关键词: 小叶黄杨, 薄层色谱, 不同部位, 环维黄杨星D

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