Research Progress on Hydrogenase Activity in Higher Plants

doi:10.19586/j.2095-2341.2022.0115

Current Biotechnology ›› 2022, Vol. 12 ›› Issue (4): 481-489.DOI: 10.19586/j.2095-2341.2022.0115

• Special Forum on Hydrogen Biomedical Science • Next Articles

Research Progress on Hydrogenase Activity in Higher Plants

Yifei SONG(), Fei XIE(), Chen MA, Xuemei MA()

Faculty of Environment and Life，Beijing University of Technology，Beijing 100124，China

Received:2022-06-30 Accepted:2022-07-03 Online:2022-07-25 Published:2022-08-10
Contact: Fei XIE,Xuemei MA

高等植物氢化酶活性研究进展

宋怡菲(), 谢飞(), 马晨, 马雪梅()

北京工业大学环境与生命学部，北京 100124

通讯作者: 谢飞,马雪梅
作者简介:宋怡菲 E-mail：S202165052@emails.bjut.edu.cn
基金资助:
军委后勤保障部开放研究重点项目(BHJ17L018)

Abstract

Abstract:

As a metalloenzyme that can catalyze hydrogen oxidation and proton reduction， hydrogenase plays a key role in the hydrogen metabolism of organisms. Previous studies have shown that hydrogen intervention has a positive impact on plant growth and stress resistance. At the same time， the phenomenon of endogenous hydrogen production in some higher plants has also been confirmed. However， little is known about the hydrogenase that catalyzes endogenous hydrogen production. Although many studies have shown that chloroplasts may be the key part of hydrogen production in higher plants， in view of the fact that many plants can still produce hydrogen during seed germination when chloroplasts have not been formed， combining that hydrogenase has evolutionary homology with mitochondrial complex Ⅰ， the hypothesis that higher plant mitochondria have hydrogenase activity was put forward and the preliminary experimental evidence of hydrogenase activity in mitochondria was summarized on the basis of summarizing the research status of hydrogenase， hoping to provide a reference for the follow-up study on the relationship between mitochondria and hydrogenase.

Key words: hydrogenase, higher plant, mitochondrion, complex Ⅰ

摘要：

氢化酶作为一种可催化氢气氧化与质子还原的金属酶，在生物体的氢代谢过程中发挥着关键作用。已有研究表明，氢气干预可对植物的生长发育和抗逆性产生积极影响，同时一些高等植物的内源性产氢现象也已得到证实，然而关于催化内源性产氢的氢化酶目前了解较少。虽然已有多项研究表明，叶绿体可能是高等植物产氢的关键部位，但是鉴于多种植物在种子萌发时仍然可以产氢，而种子萌发过程中叶绿体还没有生成，加上氢化酶在进化上与线粒体复合物Ⅰ具有同源性，在对氢化酶研究现状进行概述的基础上，提出了高等植物线粒体具有氢化酶活性的猜想，并总结了线粒体存在氢化酶活性的初步实验证据，以期为后续线粒体与氢化酶的关系研究提供参考依据。

关键词: 氢化酶, 高等植物, 线粒体, 复合物Ⅰ

CLC Number:

Yifei SONG, Fei XIE, Chen MA, Xuemei MA. Research Progress on Hydrogenase Activity in Higher Plants[J]. Current Biotechnology, 2022, 12(4): 481-489.

宋怡菲, 谢飞, 马晨, 马雪梅. 高等植物氢化酶活性研究进展[J]. 生物技术进展, 2022, 12(4): 481-489.

Figures/Tables 4

References 51

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植物种类	产氢/耗氢现象	发生部位/过程	参考文献
冬黑麦、萝卜、豌豆、番茄	产氢	种子萌发过程中	[30]
豌豆	产氢+耗氢	离体叶绿体	[31]
大麦	产氢	厌氧培养的大麦幼苗	[1]
豌豆、菠菜	产氢	离体叶绿体	[4]
水稻	产氢	水稻幼苗	[32]
拟南芥	产氢	拟南芥幼苗	[5]
苜蓿	产氢	苜蓿幼苗	[6]
猕猴桃	产氢	新鲜果肉组织	[11]
番茄	产氢	新鲜果肉组织	[13]
洋桔梗	产氢	新鲜花茎	[12]
绿豆、辣椒	产氢+耗氢	植物幼苗中纯化出的质膜	[33]

植物种类	产氢/耗氢现象	发生部位/过程	参考文献
冬黑麦、萝卜、豌豆、番茄	产氢	种子萌发过程中	[30]
豌豆	产氢+耗氢	离体叶绿体	[31]
大麦	产氢	厌氧培养的大麦幼苗	[1]
豌豆、菠菜	产氢	离体叶绿体	[4]
水稻	产氢	水稻幼苗	[32]
拟南芥	产氢	拟南芥幼苗	[5]
苜蓿	产氢	苜蓿幼苗	[6]
猕猴桃	产氢	新鲜果肉组织	[11]
番茄	产氢	新鲜果肉组织	[13]
洋桔梗	产氢	新鲜花茎	[12]
绿豆、辣椒	产氢+耗氢	植物幼苗中纯化出的质膜	[33]

研究对象	作用领域	作用效果	参考文献
拟南芥	参与植物抗生物与非生物胁迫	提高拟南芥耐盐性	[5]
紫花苜蓿	参与植物抗生物与非生物胁迫	增强苜蓿对百草枯诱导的氧化胁迫耐受性	[6]
	参与植物抗生物与非生物胁迫	增强苜蓿的镉耐受性	[8]
	参与植物抗生物与非生物胁迫	缓解苜蓿的渗透胁迫	[9]
水稻	参与植物抗生物与非生物胁迫	缓解水稻种子萌发过程中的盐胁迫	[7]
黄瓜	参与植物抗生物与非生物胁迫	缓解黄瓜植株所受干旱胁迫	[36]
猕猴桃	改善植物品质与保鲜	延缓猕猴桃采后成熟和衰老	[10]
洋桔梗	改善植物品质与保鲜	延缓洋桔梗鲜切花花瓣衰老	[12]
番茄	改善植物品质与保鲜	抑制番茄果实贮藏中亚硝酸盐的积累	[13]
小苍兰	改善植物品质与保鲜	增加叶片、花茎长度与花朵直径	[37]
番茄	参与调控植物生长发育	参与诱导番茄幼苗的侧根形成	[38]
黑麦	参与调控植物生长发育	促进黑麦种子的萌发	[39]
万寿菊	参与调控植物生长发育	调控万寿菊的不定根发育	[40]

研究对象	作用领域	作用效果	参考文献
拟南芥	参与植物抗生物与非生物胁迫	提高拟南芥耐盐性	[5]
紫花苜蓿	参与植物抗生物与非生物胁迫	增强苜蓿对百草枯诱导的氧化胁迫耐受性	[6]
	参与植物抗生物与非生物胁迫	增强苜蓿的镉耐受性	[8]
	参与植物抗生物与非生物胁迫	缓解苜蓿的渗透胁迫	[9]
水稻	参与植物抗生物与非生物胁迫	缓解水稻种子萌发过程中的盐胁迫	[7]
黄瓜	参与植物抗生物与非生物胁迫	缓解黄瓜植株所受干旱胁迫	[36]
猕猴桃	改善植物品质与保鲜	延缓猕猴桃采后成熟和衰老	[10]
洋桔梗	改善植物品质与保鲜	延缓洋桔梗鲜切花花瓣衰老	[12]
番茄	改善植物品质与保鲜	抑制番茄果实贮藏中亚硝酸盐的积累	[13]
小苍兰	改善植物品质与保鲜	增加叶片、花茎长度与花朵直径	[37]
番茄	参与调控植物生长发育	参与诱导番茄幼苗的侧根形成	[38]
黑麦	参与调控植物生长发育	促进黑麦种子的萌发	[39]
万寿菊	参与调控植物生长发育	调控万寿菊的不定根发育	[40]

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Research Progress on Hydrogenase Activity in Higher Plants

高等植物氢化酶活性研究进展

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