CRISPR/Cas9基因编辑技术在丝状真菌中的应用

doi:10.19586/j.2095-2341.2022.0038

生物技术进展 ›› 2022, Vol. 12 ›› Issue (5): 696-704.DOI: 10.19586/j.2095-2341.2022.0038

CRISPR/Cas9基因编辑技术在丝状真菌中的应用

于鲲¹(), 薛佳琪¹, 王进宽², 余永涛¹()

^1.宁夏大学农学院，银川 750021
^2.固原市原州区张易镇畜牧兽医工作站，宁夏固原 756022

收稿日期:2022-03-17 接受日期:2022-06-10 出版日期:2022-09-25 发布日期:2022-09-30
通讯作者: 余永涛
作者简介:于鲲 E-mail：yk1018120@163.com；
基金资助:
国家自然科学基金项目(31960348)

Research Progress on Application of CRISPR/Cas9 Gene Editing Technique in Filamentous Fungi

Kun YU¹(), Jiaqi XUE¹, Jinkuan WANG², Yongtao YU¹()

^1.School of Agricuture Ningxia University，Yinchuan 750021，China
^2.Animal Husbandry and Veterinary Workstation of Zhangyi Town，Yuanzhou District，Ningxia Guyuan 756022，China

Received:2022-03-17 Accepted:2022-06-10 Online:2022-09-25 Published:2022-09-30
Contact: Yongtao YU

摘要/Abstract

摘要：

随着对丝状真菌基因水平研究的不断深入，CRISPR/Cas9技术作为先进的基因编辑技术，已被广泛应用于丝状真菌的基因编辑。探究了CRISPR/Cas9系统在不同丝状真菌中的应用情况，主要从sgRNA的构建与表达、Cas9蛋白的改造与表达、不同的DNA双链断裂修复（DNA double-strand break，DSB）方式等方面进行概述，并对编辑效率、脱靶效应进行总结，旨在为今后丝状真菌中CRISPR/Cas9系统的构建及改良提供思路。

关键词: 丝状真菌, 基因编辑, CRISPR/Cas9

Abstract:

With the development of the research on filamentous fungi gene level， CRISPR/Cas9 technology is an advanced gene editing technology， which has been widely used in filamentous fungi gene editing. This article explored the application of the CRISPR/Cas9 system in different filamentous fungi. It included an overview of the construction and expression of sgRNA， the transformation and expression of Cas9 protein， and different DNA double-strand break （DSB） methods， and the editing efficiency and off-target effects were also summarized. It was expected to provided ideas for the construction and improvement of the CRISPR/Cas9 system in the future.

Key words: filamentous fungi, gene editing, CRISPR/Cas9

中图分类号:

于鲲, 薛佳琪, 王进宽, 余永涛. CRISPR/Cas9基因编辑技术在丝状真菌中的应用[J]. 生物技术进展, 2022, 12(5): 696-704.

Kun YU, Jiaqi XUE, Jinkuan WANG, Yongtao YU. Research Progress on Application of CRISPR/Cas9 Gene Editing Technique in Filamentous Fungi[J]. Current Biotechnology, 2022, 12(5): 696-704.

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CRISPR/Cas9基因编辑技术在丝状真菌中的应用

Research Progress on Application of CRISPR/Cas9 Gene Editing Technique in Filamentous Fungi

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